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PE anti-human CD275 (B7-H2, ICOSL) Antibody

Pricing & Availability
Clone
2D3 (See other available formats)
Regulatory Status
RUO
Other Names
CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
2D3_PE_072007
Human monocyte-derived dendritic cells stained with 2D3 PE
  • 2D3_PE_072007
    Human monocyte-derived dendritic cells stained with 2D3 PE
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309403 25 tests $117
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309404 100 tests $256
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Description

B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human B7H2-mIg fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kurosawa S, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:563.
Product Citations
  1. Jiao Q, et al. 2020. Br J Dermatol. 182:648. PubMed
  2. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  3. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  4. Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed
  5. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  6. Schatton T, et al. 2010. Cancer Res. 70:697. PubMed
  7. Dianzani C, et al. 2010. J Immunol. 185:3970. PubMed
  8. O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
  9. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
RRID
AB_314770 (BioLegend Cat. No. 309403)
AB_314770 (BioLegend Cat. No. 309404)

Antigen Details

Structure
Ig superfamily, B7 family, 40 kD
Distribution

B cells and dendritic cells

Function
Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
Ligand/Receptor
ICOS
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Wang S, et al. 2002. J. Biol. Chem. 96:2808.
  2. Wong SC, et al. 2003. Blood 102:1831.
Gene ID
23308 View all products for this Gene ID
UniProt
View information about CD275 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07/08/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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