PerCP/Cyanine5.5 anti-human CD49b Antibody, CD49b, P1E6-C5

Pricing & Availability

Clone: P1E6-C5 (See other available formats)
Regulatory Status: RUO
Other Names: α2 integrin, VLA-2 α chain, Integrin α2 chain
Isotype: Mouse IgG1, κ
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Product Citations: publications

P1E6-C5_PerCPCyanine55_CD49b_Antibody_101419 — Human peripheral blood platelets were stained with CD49b (clone P1E6-C5) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).

Compare all formats See PerCP/Cyanine5.5 spectral data

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359315

25 tests

$176

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359316

100 tests

$382

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Description

CD49b is a 170 kD transmembrane protein, also known as α₂ integrin, VLA-2 α chain, Integrin α₂ and GPIa. It associates with CD29 (β₁ integrin) to form VLA-2, a collagen and laminin receptor on many cell types including monocytes, platelets, activated T cells, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts. CD49b has been reported to interact with F-actin and matrix metalloproteinase 1. CD49b is a platelet alloantigen and has been associated with neonatal alloimmune thrombocytopenia. Deficiencies in this protein have been associated with hemorrhagic disorders.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HT1080 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats of this clone) include: in vitro blocking activity^1,2, immunoprecipitation³, and ELISA⁴.
RRID: AB_2820014 (BioLegend Cat. No. 359315)
AB_2820014 (BioLegend Cat. No. 359316)

Antigen Details

Structure: Integrin alpha chain family, type I membrane protein, alpha chain of heterodimeric integrin receptor, 170 kD; associates with CD29 to form collagen, laminin receptor
Distribution: Broadly expressed on many cell types including B cells, monocytes, platelets, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts
Function: With CD29; binds collagen and laminin
Ligand/Receptor: Forms heterodimer with integrin β1 (CD29) which is a receptor for laminin and collagen
Biology Area: Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Kaplan C, et al. 1991. Br. J. Haematol. 78:425.
2. Kiefel V, et al. 1991. Vox Sang. 60:244.
3. Nieuwenhuis HK, et al. 1985. Nature 318:470.
4. Takada Y and Helmer ME. 1989. J. Cell Biol. 109:397.
Gene ID: 3673 View all products for this Gene ID
UniProt: View information about CD49b on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD49b Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD49b	P1E6-C5	FC,IP,ELISA
FITC anti-human CD49b	P1E6-C5	FC
PE anti-human CD49b	P1E6-C5	FC
APC anti-human CD49b	P1E6-C5	FC
TotalSeq™-A0371 anti-human CD49b	P1E6-C5	PG
PerCP/Cyanine5.5 anti-human CD49b	P1E6-C5	FC
PE/Cyanine7 anti-human CD49b	P1E6-C5	FC
TotalSeq™-C0371 anti-human CD49b	P1E6-C5	PG
TotalSeq™-B0371 anti-human CD49b	P1E6-C5	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10/14/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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