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PerCP/Cyanine5.5 anti-human TIGIT (VSTM3) Antibody

Pricing & Availability
Clone
A15153G (See other available formats)
Regulatory Status
RUO
Other Names
T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A15153G_PerCPCy5.5_TIGIT_Antibody_1_120516
Human peripheral blood leukocytes were stained with CD3 APC and TIGIT (clone A15153G) PerCP/Cyanine5.5 (left), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the lymphocyte population.
  • A15153G_PerCPCy5.5_TIGIT_Antibody_1_120516
    Human peripheral blood leukocytes were stained with CD3 APC and TIGIT (clone A15153G) PerCP/Cyanine5.5 (left), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Data shown was gated on the lymphocyte population.
Compare all formats See PerCP/Cyanine5.5 spectral data
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372717 25 tests $171
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372718 100 tests $429
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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human TIGIT.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional reported applications (for the relevant formats) include: Blocking1.

Application References

(PubMed link indicates BioLegend citation)
  1. Stamm H, et al. 2018. Oncogene. Pubmed
Product Citations
  1. Ogura H, et al. 2022. Nat Commun. 13:7063. PubMed
  2. Mohammad TAM, et al. 2023. Int J Rheum Dis. 26:740. PubMed
  3. Tang W, et al. 2019. Oncoimmunology. 8:e1593807. PubMed
  4. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
RRID
AB_2632933 (BioLegend Cat. No. 372717)
AB_2632933 (BioLegend Cat. No. 372718)

Antigen Details

Structure
26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
Distribution

Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.

Function
Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
Ligand/Receptor
CD155 (PVR), CD112 (PVRL2, NECTIN-2).
Cell Type
NK cells, T cells, Tfh, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923.

Gene ID
201633 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All TIGIT (VSTM3) Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human TIGIT (VSTM3) A15153G FC,Block
APC/Fire™ 750 anti-human TIGIT (VSTM3) A15153G FC
APC anti-human TIGIT (VSTM3) A15153G FC
PE anti-human TIGIT (VSTM3) A15153G FC
Brilliant Violet 421™ anti-human TIGIT (VSTM3) A15153G FC
Brilliant Violet 605™ anti-human TIGIT (VSTM3) A15153G FC
PE/Dazzle™ 594 anti-human TIGIT (VSTM3) A15153G FC
PerCP/Cyanine5.5 anti-human TIGIT (VSTM3) A15153G FC
PE/Cyanine7 anti-human TIGIT (VSTM3) A15153G FC
Ultra-LEAF™ Purified anti-human TIGIT (VSTM3) A15153G FC
Biotin anti-human TIGIT (VSTM3) A15153G FC
Alexa Fluor® 647 anti-human TIGIT (VSTM3) A15153G FC
TotalSeq™-A0089 anti-human TIGIT (VSTM3) A15153G PG
TotalSeq™-B0089 anti-human TIGIT (VSTM3) A15153G PG
TotalSeq™-C0089 anti-human TIGIT (VSTM3) A15153G PG
KIRAVIA Blue 520™ anti-human TIGIT (VSTM3) A15153G FC
APC/Cyanine7 anti-human TIGIT (VSTM3) A15153G FC
Brilliant Violet 510™ anti-human TIGIT (VSTM3) A15153G FC
Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody A15153G FC
TotalSeq™-D0089 anti-human TIGIT (VSTM3) A15153G PG
Brilliant Violet 711™ anti-human TIGIT (VSTM3) A15153G FC
PE/Fire™ 640 anti-human TIGIT (VSTM3) A15153G FC
PE/Fire™ 810 anti-human TIGIT (VSTM3) A15153G FC
PE/Cyanine5 anti-human TIGIT (VSTM3) A15153G FC
PerCP/Fire™ 806 anti-human TIGIT (VSTM3) A15153G FC
PerCP/Fire™ 780 anti-human TIGIT (VSTM3) A15153G FC
Spark Red™ 718 anti-human TIGIT (VSTM3) (Flexi-Fluor™) A15153G FC
PE/Fire™ 700 anti-human TIGIT (VSTM3) A15153G FC
Go To Top Version: 1    Revision Date: 12/28/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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