Spark Red™ 718 anti-human TIGIT (VSTM3) (Flexi-Fluor™) Antibody, TIGIT (VSTM3), A15153G

Pricing & Availability

Clone: A15153G (See other available formats)
Regulatory Status: RUO
Other Names: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Isotype: Mouse IgG2a, κ
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285002

50 µg

$375

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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human TIGIT.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional reported applications (for the relevant formats) include: Blocking¹.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3106288 (BioLegend Cat. No. 285002)

Antigen Details

Structure: 26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
Distribution: Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.
Function: Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
Ligand/Receptor: CD155 (PVR), CD112 (PVRL2, NECTIN-2).
Cell Type: NK cells, T cells, Tfh, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
Molecular Family: Adhesion Molecules, Immune Checkpoint Receptors
Antigen References: 1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923.
Gene ID: 201633 View all products for this Gene ID
UniProt: View information about TIGIT on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All TIGIT (VSTM3) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human TIGIT (VSTM3)	A15153G	FC,Block
APC/Fire™ 750 anti-human TIGIT (VSTM3)	A15153G	FC
APC anti-human TIGIT (VSTM3)	A15153G	FC
PE anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 421™ anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 605™ anti-human TIGIT (VSTM3)	A15153G	FC
PE/Dazzle™ 594 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Cyanine5.5 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Cyanine7 anti-human TIGIT (VSTM3)	A15153G	FC
Ultra-LEAF™ Purified anti-human TIGIT (VSTM3)	A15153G	FC
Biotin anti-human TIGIT (VSTM3)	A15153G	FC
Alexa Fluor® 647 anti-human TIGIT (VSTM3)	A15153G	FC
TotalSeq™-A0089 anti-human TIGIT (VSTM3)	A15153G	PG
TotalSeq™-B0089 anti-human TIGIT (VSTM3)	A15153G	PG
TotalSeq™-C0089 anti-human TIGIT (VSTM3)	A15153G	PG
KIRAVIA Blue 520™ anti-human TIGIT (VSTM3)	A15153G	FC
APC/Cyanine7 anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 510™ anti-human TIGIT (VSTM3)	A15153G	FC
Brilliant Violet 785™ anti-human TIGIT (VSTM3) Antibody	A15153G	FC
TotalSeq™-D0089 anti-human TIGIT (VSTM3)	A15153G	PG
Brilliant Violet 711™ anti-human TIGIT (VSTM3)	A15153G	FC
PE/Fire™ 640 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Fire™ 810 anti-human TIGIT (VSTM3)	A15153G	FC
PE/Cyanine5 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Fire™ 806 anti-human TIGIT (VSTM3)	A15153G	FC
PerCP/Fire™ 780 anti-human TIGIT (VSTM3)	A15153G	FC
Spark Red™ 718 anti-human TIGIT (VSTM3) (Flexi-Fluor™)	A15153G	FC
PE/Fire™ 700 anti-human TIGIT (VSTM3)	A15153G	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02/06/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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