PerCP/Cyanine5.5 anti-mouse CD11a Antibody, CD11a, 2D7

Pricing & Availability

Clone: 2D7 (See other available formats)
Regulatory Status: RUO
Other Names: αL integrin, LFA-1 α subunit, Ly-15, Ly-21, ITGAL
Isotype: Rat IgG2a, κ
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Product Citations: publications

2D7_PERCP-CYA55_CD11a_FC_122923 — C57BL/6 splenocytes were stained with anti-mouse CD11a (clone 2D7) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).

Compare all formats See PerCP/Cyanine5.5 spectral data

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162907

25 µg

$120

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162908

100 µg

$295

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Description

CD11a is a 180 kD glycoprotein, also known as α_L integrin, LFA-1 α, Ly-15, or Ly-21. It is a member of the integrin family, primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes. In association with CD18, the CD11a/CD18 complex forms LFA-1. CD11a plays an important role in intercellular adhesion and costimulation by binding its ligands, ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3097463 (BioLegend Cat. No. 162907)
AB_3097463 (BioLegend Cat. No. 162908)

Antigen Details

Structure: Integrin, associated with integrin CD18, 180 kD
Distribution: Lymphocytes, granulocytes, monocytes, macrophages
Function: Cellular adhesion
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)
Cell Type: Granulocytes, Lymphocytes, Macrophages, Monocytes
Biology Area: Cell Biology, Immunology
Molecular Family: CD Molecules
Antigen References: 1. .Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.
Gene ID: 16408 View all products for this Gene ID
UniProt: View information about CD11a on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD11a Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD11a Antibody	2D7	FC,IHC-F
Alexa Fluor® 488 anti-mouse CD11a	2D7	FC
APC/Fire™ 750 anti-mouse CD11a	2D7	FC
PerCP/Cyanine5.5 anti-mouse CD11a	2D7	FC
Brilliant Violet 421™ anti-mouse CD11a	2D7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/29/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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