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Purified anti-ALDH1L1 Antibody

Pricing & Availability
Clone
N103/39 (See other available formats)
Regulatory Status
RUO
Other Names
FDH
Isotype
Mouse IgG1, κ
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Product Citations
publications
N103-39_Purified_ALDH1L1_Antibody_1_112018
Western blot of purified anti-ALDH1L1 antibody (clone N103/39). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 5 µg of mouse liver lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N103-39_Purified_ALDH1L1_Antibody_1_112018
    Western blot of purified anti-ALDH1L1 antibody (clone N103/39). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 5 µg of mouse liver lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N103-39_Purified_ALDH1L1_Antibody_2_122123
    IHC staining of Purified anti-ALDH1L1 (clone N103/39) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of Purified anti-ALDH1L1 (clone N103/39) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
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856801 25 µg $83
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856802 100 µg $165
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Description

Aldehyde dehydrogenase family 1 member L1 (ALDH1L1) was originally discovered as an enzyme that catalyzes the conversion of 10-formyltetrahydrofolate to tetrahydrofolate, with NADPH and CO2 as by products. ALDH1L1 is most exclusively expressed by astroglial cells1 implicating these cells as the major components of folate-dependent formate oxidation in the brain.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 5.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant) formats include immunocytochemistry3 and immunohistochemistry on frozen tissue sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Zuo H, et al. 2018. J Neurosci. 38(9):2359 (IHC-F)
  2. Kidana K, et al. 2018. EMBO Mol Med. 10:e8184 (WB)
  3. Tian E, et al. 2016. Cell Reports. 16:781 (ICC)
RRID
AB_2783463 (BioLegend Cat. No. 856801)
AB_2783463 (BioLegend Cat. No. 856802)

Antigen Details

Structure
ALDH1L1 is a 902 amino acid protein with an apparent molecular mass of ~ 99 kD.
Distribution

Tissue Distribution: Brain, Liver, Colon, and Kidney.
Cellular Distribution: Extracellular, cytosol, and plamsa membrane.

Function
ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate to tetrahydrofolate.
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Neymerer V, et al. 1997. Brain Res. 766(1-2): 195
  2. Brandebura AN, et al. 2018. Dev Neurobiol. doi: 10.1002/dneu.22633.
  3. Garwood CJ, et al. 2017. Neuropathol Appl Neurobiol. 43(4):281
Gene ID
10840 View all products for this Gene ID 107747 View all products for this Gene ID
UniProt
View information about ALDH1L1 on UniProt.org
Go To Top Version: 2    Revision Date: 12/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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