- Clone
- 2E1.E9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Glial fibrillary acid protein
- Isotype
- Mouse IgG2b
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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644701 | 25 µg | $124 | ||||
644702 | 100 µg | $293 |
GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Bovine spinal cord homogenate
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IHC-F, IHC-P - Verified
FC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.25 - 1.0 µg antibody per mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemical staining of frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For Immunohistochemistry, Citrate buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 is recommended.
-
Application References
(PubMed link indicates BioLegend citation) -
- McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221.
- Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
- Product Citations
-
- RRID
-
AB_2109791 (BioLegend Cat. No. 644701)
AB_2109791 (BioLegend Cat. No. 644702)
Antigen Details
- Structure
- Around 50 kD
- Distribution
-
Only expressed in astrocytes in the central nervous system.
- Function
- Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
- Cell Type
- Astrocytes
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- 2670 View all products for this Gene ID
- UniProt
- View information about GFAP on UniProt.org
Related FAQs
Other Formats
View All GFAP Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GFAP | 2E1.E9 | WB,ICC,IHC-F,IHC-P,FC |
Alexa Fluor® 488 anti-GFAP | 2E1.E9 | IHC-F,ICC,IHC-P,SB |
Alexa Fluor® 647 anti-GFAP | 2E1.E9 | IHC-F,IHC-P,SB |
Alexa Fluor® 594 anti-GFAP | 2E1.E9 | IHC-F,IHC-P,SB |
Brilliant Violet 421™ anti-GFAP | 2E1.E9 | IHC-F,ICC,IHC-P |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-GFAP
Total cell lysate from HeLa (negative control), U87-MG and t... U251 cells were fixed with 4% paraformaldehyde (PFA) for ten... C57BL/6 mouse frozen brain section was fixed with 4% parafor... IHC staining of Purified anti-GFAP (clone 2E1.E9) on formali... IHC staining of purified anti-GFAP (clone 2E1.E9) on formali... -
Alexa Fluor® 488 anti-GFAP
C57BL/6 mouse frozen brain section was fixed with 4% parafor... Day-three cultured postnatal C57BL/6 mouse brain cells were ... IHC staining of Alexa Fluor® 488 anti-GFAP (clone 2E1.E9) on... -
Alexa Fluor® 647 anti-GFAP
C57BL/6 mouse frozen cerebellum section was fixed with 4% pa... Human paraffin-embedded cerebellum tissue slices were prepar... Human paraffin-embedded cerebellum tissue slices were prepar... -
Alexa Fluor® 594 anti-GFAP
C57BL/6 mouse frozen cerebellum section was fixed with 4% pa... IHC staining of Alexa Fluor® 594 anti-GFAP (clone 2E1.E9) on... -
Brilliant Violet 421™ anti-GFAP
Day-three cultured postnatal C57BL/6 mouse brain cells were ... C57BL/6 mouse frozen brain tissue was fixed with 4% paraform... Human paraffin-embedded human cerebellum tissue slices were ...
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