- Clone
- IA6-2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ig delta chain C region
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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348235 | 100 µg | $83 |
IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IgD
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections4 and spatial biology (IBEX)5,6.
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Chen K, et al. 2009. Nat. Immunol. 10:889.
- Lee CH, et al. 2005. J. Exp. Med. 203:63.
- Sutter JA, et al. 2008. Clin. Immunol. 126:282.
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2563775 (BioLegend Cat. No. 348235)
Antigen Details
- Structure
- Exists in a transmembranal and a soluble form
- Distribution
-
Naïve B cells
- Function
- Antigen binding, B cell activation
- Interaction
- The CD79a/CD79b heterodimer
- Cell Type
- B cells
- Biology Area
- Immunology
- Antigen References
-
1. Geisberger R, et al. 2006. Immunology 118:429.
2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789.
3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32. - Gene ID
- 3495 View all products for this Gene ID
- UniProt
- View information about IgD on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
PerCP anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 605™ anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 FI... -
Alexa Fluor® 700 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
Purified anti-human IgD
Human periperhal blood lymphocytes stained with purified IA6... -
PE anti-human IgD
Human periperhal blood lymphocytes stained with IA6-2 PE and... -
Biotin anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 (c... -
FITC anti-human IgD
-
PerCP/Cyanine5.5 anti-human IgD
Human peripheral blood lymphocytes stained with CD19 APC and... -
PE/Cyanine7 anti-human IgD
-
Alexa Fluor® 488 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 Br... Confocal image of human lymph node sample acquired using the... -
APC/Cyanine7 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 Br... -
Brilliant Violet 510™ anti-human IgD
Human peripheral blood lymphocytes were stained with IgD (cl... -
APC anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
Pacific Blue™ anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
Brilliant Violet 421™ anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 AP... -
Purified anti-human IgD (Maxpar® Ready)
Human PBMCs stained with 147Sm-anti-CD20 (2H7) and 146Nd-ant... -
PE/Dazzle™ 594 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 AP... -
APC/Fire™ 750 anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 FI... -
Brilliant Violet 785™ anti-human IgD
Human peripheral blood lymphocytes were stained with CD19 PE... -
TotalSeq™-A0384 anti-human IgD
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TotalSeq™-C0384 anti-human IgD
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TotalSeq™-B0384 anti-human IgD
-
PE/Cyanine5 anti-human IgD
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0384 anti-human IgD
-
Spark UV™ 387 anti-human IgD
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human IgD
Typical results from human peripheral blood lymphocytes stai...
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