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Purified anti-human TSLP Antibody

Pricing & Availability
Clone
15B11.3 (See other available formats)
Regulatory Status
RUO
Other Names
Thymic stromal lymphopoietin (TSLP)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
15B11_040408
Human TSLP Direct ELISA (Clone 15B11)
  • 15B11_040408
    Human TSLP Direct ELISA (Clone 15B11)
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512202 500 µg $317
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Description

Thymic Stromal Lymphopoietin (TSLP) is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs). TSLP activated DCs primed naïve T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFα) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergic inflammation. Recent studies revealed that expression of TSLP has a direct link to the pathogenesis of allergic inflammation such as atopic dermatitis and asthma via activating dendritic cells and mast cells which trigger inflammatory Th2 response characterized by high TNF-alpha and little IL-10 production.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human TSLP
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of TSLP recombinant protein ranging from 200 to 3.1 ng/ml are recommended for each ELISA plate.

Application Notes

ELISA Capture: Purified and Ultra-LEAF™ purified 15B11.3 antibody are useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5159 antibody (Cat. No. 515903) as the detecting antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Yeh CY, et al. 2013. PLoS One. 8:e55472. (Neut) PubMed
RRID
AB_940352 (BioLegend Cat. No. 512202)

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Liu YJ. 2007. J. Allergy Clin. Immunol. 120:238.
2. Nagata Y. 2007. Int. Arch. Allergy Immunol. 144:305.
3. Jiang Q, et al. 2007 BMC Immunol. 8:11.
4. Kato A. et al. 2007. J. Immunol. 179:1080.
5. Rochman I, et al. 2007 J. Immunol. 178:6720.
6. Reche PA, et al. 2001. J. Immunol. 167:336.
7. Pandey A, et al. 2000. Nat. Immunol. 1:59.
8. Park LS, et al. 2000. J Exp. Med. 192:659.

Gene ID
85480 View all products for this Gene ID
UniProt
View information about TSLP on UniProt.org
Go To Top Version: 2    Revision Date: 01/06/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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