Purified anti-mouse CD335 NKp46 Maxpar Ready Antibody anti-CD335

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Clone: 29A1.4 (See other available formats)
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Rat IgG2a, κ
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Product Citations: publications

29A1.4_Purified_CD335_Cytof_060414.jpg — Mouse splenocytes stained with 153Eu-anti-CD335 (29A1.4) and 170Er-anti-CD161 (NK1.1) (PK136). Data provided by DVS Sciences.

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137625

100 µg

$171

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Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1,2 and in vitro activation of NK cells¹.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563744 (BioLegend Cat. No. 137625)

Antigen Details

Structure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.
Gene ID: 17086 View all products for this Gene ID
UniProt: View information about CD335 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD335 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 510™ anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 711™ anti-mouse CD335 (NKp46)	29A1.4	FC
PE anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Cyanine7 anti-mouse CD335 (NKp46)	29A1.4	FC
Purified anti-mouse CD335 (NKp46)	29A1.4	FC,IHC-F
FITC anti-mouse CD335 (NKp46)	29A1.4	FC
APC anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Cyanine5.5 anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 421™ anti-mouse CD335 (NKp46)	29A1.4	FC
Biotin anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 605™ anti-mouse CD335 (NKp46)	29A1.4	FC
Purified anti-mouse CD335 (NKp46) (Maxpar® Ready)	29A1.4	FC,CyTOF®
Alexa Fluor® 647 anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Dazzle™ 594 anti-mouse CD335 (NKp46)	29A1.4	FC
APC/Fire™ 750 anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 650™ anti-mouse CD335 (NKp46)	29A1.4	FC
TotalSeq™-A0184 anti-mouse CD335 (NKp46)	29A1.4	PG
Brilliant Violet 785™ anti-mouse CD335 (NKp46)	29A1.4	FC
Ultra-LEAF™ Purified anti-mouse CD335 (NKp46)	29A1.4	FC,IHC-F
TotalSeq™-B0184 anti-mouse CD335 (NKp46)	29A1.4	PG
TotalSeq™-C0184 anti-mouse CD335 (NKp46)	29A1.4	PG
APC/Cyanine7 anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Cyanine5 anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Fire™ 806 anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Fire™ 780 anti-mouse CD335 (NKp46)	29A1.4	FC
Spark Red™ 718 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC
Spark Blue™ 574 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC
Spark Blue™ 550 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

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