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Purified anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
8.1.1 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, gp38, stromal cell marker
Isotype
Syrian Hamster IgG
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Product Citations
publications
1_8dot1dot1_Pure_061708.jpg
TE-71 cells stained with purified 8.1.1, followed by anti-Syrian hamster IgG FITC
  • 1_8dot1dot1_Pure_061708.jpg
    TE-71 cells stained with purified 8.1.1, followed by anti-Syrian hamster IgG FITC
  • 2_8dot1dot1_pure_Podoplanin_Antibody_2_103018
    Fresh, frozen mouse spleen was stained with purified Podoplanin clone 8.1.1 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
  • 3_58_Mouse_Lymph_Node_gp38_SIRPa
    Mice were injected subcutaneously with sheep red blood cells in a volume of 25 µl per site on days 0 and 4 and harvested on day 11. Confocal image of C57BL/6 mouse lymph node acquired using the IBEX method of highly multiplexed antibody-based imaging: gp38 (purple) in Cycle 2 and SIRPα (cyan) in Cycle 5. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
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127401 50 µg $71
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127402 500 µg $282
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
  2. Farr AG, et al. 1992. J. Exp. Med. 176:1477.
  3. Bekiaris V, et al. 2008. J. Immunol. 180:6768.
  4. Algars A, et al. 2011. Blood 117:4387. PubMed
  5. Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
  6. Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
  7. Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Kida H, et al. 2017. Sci Rep. 7:12032. PubMed
  3. Mahamud MR, et al. 2023. Microcirculation. 30:e12787. PubMed
  4. Lütge M, et al. 2023. Nat Immunol. . PubMed
  5. Dubrot J, et al. 2018. Life Sci Alliance. 1:e201800164. PubMed
  6. Kretschmer S, et al. 2013. PLoS One. 8:e55201. PubMed
  7. Reis V, et al. 2012. Immunobiology. 217:831. PubMed
  8. Gao J, et al. 2017. Oncol Lett. 14:2954. PubMed
  9. Mahamud MR, et al. 2019. Development. 146:dev184218. PubMed
  10. Kajiwara K, et al. 2021. BMC Nephrol. 22:3. PubMed
  11. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  12. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  13. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  14. Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
  15. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  16. Le HQ, et al. 2021. EMBO Rep. 22:e52785. PubMed
  17. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  18. Molica F,et al. 2017. Sci Rep.. 10.1038/s41598-017-14130-4. PubMed
  19. ålgars A, et al. 2011. Blood. 117:4387. PubMed
  20. Chihara N, et al. 2018. Nature. 558:454. PubMed
  21. Munir H, et al. 2021. Nat Commun. 0.974305556. PubMed
  22. Kwok T, et al. 2022. Front Aging. 3:838943. PubMed
  23. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  24. Kraft JC, et al. 2018. J Drug Target. 26:494. PubMed
  25. Tamburini BAJ, et al. 2019. Front Immunol. 10:1036. PubMed
  26. Takeuchi Y, et al. 2021. Sci Rep. 11:18679. PubMed
  27. Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
  28. Mece O, et al. 2022. Nat Commun. 13:2760. PubMed
  29. Choi SY, et al. 2020. Nat Commun. 0.81875. PubMed
  30. Nagashima K, et al. 2017. Biochem Biophys Res Commun. . 10.1016/j.bbrc.2017.09.004. PubMed
RRID
AB_1089186 (BioLegend Cat. No. 127401)
AB_1089186 (BioLegend Cat. No. 127402)

Antigen Details

Structure
43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Cell Type
Epithelial cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.

Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 3    Revision Date: 04/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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