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PE anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
8.1.1 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, gp38, stromal cell marker
Isotype
Syrian Hamster IgG
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Product Citations
publications
8_1_1_PE_081908.jpg
TE-71, mouse thymic epithelial stromal cell line, stained with 8.1.1 PE
  • 8_1_1_PE_081908.jpg
    TE-71, mouse thymic epithelial stromal cell line, stained with 8.1.1 PE
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127407 50 µg $134
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127408 200 µg $408
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
  2. Farr AG, et al. 1992. J. Exp. Med. 176:1477.
  3. Bekiaris V, et al. 2008. J. Immunol. 180:6768.
  4. Algars A, et al. 2011. Blood 117:4387. PubMed
  5. Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
  6. Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
  7. Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Du Y, et al. 2022. FASEB J. 36:e22248. PubMed
  2. Akasaka H, et al. 2023. JCI Insight. 8:. PubMed
  3. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  4. Zinselmeyer B, et al. 2013. J Exp Med. 210:757. PubMed
  5. Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
  6. Novkovic M, et al. 2020. Cell Reports. 30(3):893-904.e6.. PubMed
  7. Luo R, et al. 2021. Theranostics. 11:117. PubMed
  8. Chauhan A, et al. 2020. Nat Commun. 11:1939. PubMed
  9. Liu M, et al. 2020. Nature. 587:115. PubMed
  10. Mkrtichyan M, et al. 2012. J Immunol. 189:2450. PubMed
  11. Hara T, et al. 2012. J Immunol. 189:1577. PubMed
  12. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  13. Bresson L, et al. 2018. Development. 145. PubMed
  14. Sun Q, et al. 2015. Bone Res. 3: 15026. PubMed
  15. Sullivan B, et al. 2015. PLoS Pathog. 11:1004588. PubMed
  16. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  17. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
  18. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  19. Baasch S, et al. 2021. Cell. . PubMed
  20. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  21. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  22. Pezoldt J, et al. 2022. Nat Commun. 13:7227. PubMed
  23. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  24. Peranzoni E, et al. 2018. Proc Natl Acad Sci U S A. 115:E4041. PubMed
  25. Tang C, et al. 2021. Theranostics. 11:9791. PubMed
  26. Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed
  27. Karpus ON, et al. 2019. Cell Rep. 26:3698. PubMed
  28. Ito K, et al. 2014. Proc Natl Acad Sci U S A. 111:3080. PubMed
  29. Francipane MG, et al. 2020. Am J Pathol. 190:252. PubMed
  30. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  31. Kataru RP, et al. 2022. Front Aging. 3:864860. PubMed
  32. Mishra A, et al. 2016. J Immunol. 197: 2828 - 2837. PubMed
  33. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  34. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  35. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  36. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
  37. Goldberg AR, et al. 2022. Front Physiol. 13:1021038. PubMed
  38. Bieniasz-Krzywiec P, et al. 2021. STAR Protoc. 2:100481. PubMed
  39. Othy S, et al. 2013. J Immunol. 190:4535. PubMed
  40. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_2161928 (BioLegend Cat. No. 127407)
AB_2161928 (BioLegend Cat. No. 127408)

Antigen Details

Structure
43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Cell Type
Epithelial cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.

Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/03/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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