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Purified anti-RUNX1 Antibody

Pricing & Availability
Clone
1C5B16 (See other available formats)
Regulatory Status
RUO
Other Names
Runt-related transcription factor 1, Acute myeloid leukemia 1 protein (AML-1), Polyomavirus enhancer-binding protein 2 alpha B subunit (PEBP2-alpha B), SL3/AKV core-binding factor alpha B subunit
Isotype
Mouse IgG2a, κ
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Product Citations
publications
1C5B16_Purified_RUNX1_1_WB_051314
Western blot analysis of Jurkat (lane 1), THP-1 (lane 2), Hela (lane 3) and Raw264.7 (lane 4) cells using anti-RUNX1 antibody (1C5B16). GAPDH antibody (poly6314) was used as loading control.
  • 1C5B16_Purified_RUNX1_1_WB_051314
    Western blot analysis of Jurkat (lane 1), THP-1 (lane 2), Hela (lane 3) and Raw264.7 (lane 4) cells using anti-RUNX1 antibody (1C5B16). GAPDH antibody (poly6314) was used as loading control.
  • 1C5B16_Purified_RUNX1_2_IF_021814.jpg
    Jurkat cells were stained with purified anti-RUNX1(clone 1C5B16) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
  • 1C5B16_Purified_RUNX1_3_IP_021814.jpg
    Immunoprecipitation of RUNX1 from Jurkat cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-RUNX1 antibody (clone 1C5B16, lane 4). Western blot was performed using anti-RUNX1 antibody (clone 1C5B16).
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659302 100 µg $265
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Description

RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding complex with CBFB. RUNX1 was first identified as a RUNX1-ETO fusion protein in acute myeloid leukaemia (AML) and is frequently mutated in AML and myelodysplastic syndrome due to chromosomal translocation. RUNX1-deficient mice fail to generate hematopoietic stem cells. RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. Impaired differentiation of megakaryocytes and decreased circulating platelet count was observed in the absence of RUNX1. These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human RUNX1 recombinant protein (191-350 aa) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per mL. For immunoprecipitation, the suggested use of this reagent is 10 - 30 μg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

This product weakly reacts with Mouse.

Product Citations
  1. Hübschmann V, et al. 2022. STAR Protoc. 3:101866. PubMed
  2. Canu G, et al. 2020. Genome Biol. 0.984027778. PubMed
  3. Li M, et al. 2021. Nat Commun. 12:420. PubMed
  4. Takao S, et al. 2021. eLife. 10:00. PubMed
  5. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
RRID
AB_2563194 (BioLegend Cat. No. 659302)

Antigen Details

Structure
453 amino acids encode a protein with a predicted MW of 48.7 kD. Contains a Runt domain responsible for DNA binding.
Distribution

Nucleus.

Function
RUNX1 is a transcription factor that plays crucial roles in hematopoiesis, hair follicle development, neurogenesis, chondrogenesis, and leukemogenesis.
Interaction
Interacts with TLE1, ELF1, ELF2, ELF4, SPI1, KAT6A, KAT6B, YAP1, and CDK6. Forms a heterodimer with CBFB.
Cross
Human, Cross-Reactivity: Mouse (weak)
Cell Type
B cells, Neural Stem Cells, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Antigen References

1. Goyama S, et al. 2013. J. Clin. Invest. 123:3876.
2. Wang J, et al. 2013. PLoS One. 18:e74255.
3. Taniuchi I, et al. 2012. EMBO J. 31:4098.
4. Neel BG, et al. 2012. Genes Dev. 26:1520.
5. Della Gatta G, et al. 2012. Nat. Med. 18:436.
6. Hoi CS, et al. 2010. Mol. Cell Biol. 30:2518.

Gene ID
861 View all products for this Gene ID
UniProt
View information about RUNX1 on UniProt.org
Go To Top Version: 5    Revision Date: 08/15/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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