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Purified anti-SARS-CoV-2 Nucleocapsid Antibody

Pricing & Availability
Clone
A20087F (See other available formats)
Regulatory Status
RUO
Other Names
Nucleocapsid phosphoprotein, N, Nucleoprotein
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A20087F_PURE_Nucleocapsid_Antibody_1_102220
Whole cell extracts (15 µg total protein) from 293E cells untransfected (-) or transfected with SARS-CoV-2 nucleocapsid (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • A20087F_PURE_Nucleocapsid_Antibody_1_102220
    Whole cell extracts (15 µg total protein) from 293E cells untransfected (-) or transfected with SARS-CoV-2 nucleocapsid (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • A20087F_PURE_Nucleocapsid_Antibody_2_102220
    Recombinant nucleocapsid protein coated onto Costar™ 96-well, high binding assay plate at 2 µg/mL was incubated with a dilution series of purified anti-SARS-CoV-2 nucleocapsid antibody (clone A20087F). Bound antibodies were detected with HRP-goat anti-mouse IgG antibody (Cat. No. 405306) followed by TMB substrate solution. Absorbance was measured at 450 nm.
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940901 25 µg $112
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940902 100 µg $335
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Description

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a single stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic in 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins.

The SARS-CoV-2 Nucleocapsid (N) is an abundant protein, that binds to the viral RNA and helps stabilize it. It has been proposed that N protein forms bimolecular condensates that both regulate viral replication and modulate the host response to the virus following infection. N protein is a major antigen that the host immune response is directed to following SARS CoV-2 infection making it a promising candidate for vaccine development and drug therapy.

Product Details
Technical data sheet

Product Details

Verified Reactivity
SARS-CoV-2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SARS-CoV-2 nucleocapsid recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For the Direct ELISA application, a concentration of 2.5 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SARS-CoV-2 is 90% homologous to SARS-CoV. Cross reactivity of anti-SARS-CoV-2 nucleocapsid antibody (clone A20087F) with SARS-CoV has not been tested.

RRID
AB_2888743 (BioLegend Cat. No. 940901)
AB_2888743 (BioLegend Cat. No. 940902)

Antigen Details

Structure
SARS-CoV-2 is a 419 amino acid protein with an expected molecular weight of 46 kD.
Distribution

Viral nucleoprotein, host cell Golgi apparatus

Function
RNA binding, transcription regulation
Biology Area
COVID-19
Antigen References
  1. Zeng W, et al. 2020. Biochem Biophys Res Commun. 527:618-623.
  2. Yoshimoto FK. 2020. Protein J. 39:198-216.
  3. Cascarina SM & Ross ED. 2020. FASEB J. fj.202001351.
Gene ID
43740575 View all products for this Gene ID
UniProt
View information about Viral Protein on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/26/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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