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Purified anti-SLC1A5 Antibody

Pricing & Availability
Clone
W19125A (See other available formats)
Regulatory Status
RUO
Other Names
Solute carrier family 1 member 5, Sodium-dependent neutral amino acid transporter type 2, Neutral amino acid transporter B(0), ATB(0), Baboon M7 virus receptor, M7V1, RD114/simian type D retrovirus receptor, RDR, RDRC, ASCT2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_W19125A_PURE_SLC1A5_Antibody_WB_112922
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-SLC1A5 (clone W19125A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • A_W19125A_PURE_SLC1A5_Antibody_WB_112922
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-SLC1A5 (clone W19125A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • B_W19125A_PURE_SLC1A5_Antibody_IHC_1112922
    IHC staining of purified anti-SLC1A5 (clone W19125A) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated with 10 μg/mL of primary antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (magenta) (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. Scale bar: 50 μm
  • C_W19125A_PURE_SLC1A5_Antibody_ICC_112922
    ICC staining of purified anti-SLC1A5 (clone W19125A) on HeLa cells. The cells were fixed with 4% paraformaldehyde, permeabilized with methanol and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (red) (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slides were mounted with ProLong™ Gold Antifade Mountant. Scale bar: 50 μm
  • D_W19125A_PURE_SLC1A5_Antibody_ICFC_112922.
    The human T lymphoblast cell line Jurkat was fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), and intracellularly stained with 0.25 µg of purified anti- SLC1A5 (clone W19125A) (filled histogram) or 0.25 µg of purified rat IgG2a, κ isotype control (open histogram, dashed line) (Cat. No. 400501) followed by PE goat anti-rat IgG (Cat. No. 405406).
  • E_W19125A_PURE_SLC1A5_Antibody_IP_102022
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype control (Cat. No. 400502) or purified anti-SLC1A5 (clone W19125A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of SLC1A5. Lane M: Molecular weight marker
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608051 25 µg $118
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608052 100 µg $293
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Description

Solute carrier family 1 member 5 (SLC1A5 or ASCT2) belongs to the SLC1 transporter family, which is used for exchanging neutral amino acids, especially glutamine uptake. SLC1A5 on the cytosolic membrane transports glutamine inwardly to regulate cellular energy metabolism, biosynthesis, cell proliferation, and signal transduction. In hematopoietic stem cells, SLC1A5 is necessary for erythroid cell differentiation. During placenta development, SLC1A5 serves as the receptor of syncytin-1 to stimulate trophoblast syncytialization. SLC1A5 is highly expressed in multiple cancers by positively regulating c-Myc, mTOR, and MAPK-PI3K signaling pathways to support tumor cell chemoresistance and cell growth. In addition, an SLC1A5 isoform variant induced by HIF-2α is expressed in mitochondria, that enhances metabolism and promotes drug resistance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human SLC1A5 (400-541)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product does not cross react with mouse in western blotting.

RRID
AB_2927964 (BioLegend Cat. No. 608051)
AB_2927964 (BioLegend Cat. No. 608052)

Antigen Details

Structure
SLC1A5 is a 541 amino-acid protein with a predicted molecular weight of 56 kD.
Distribution

SLC1A5 is expressed in placenta, lung, skeletal muscle, intestine, kidney, testis, T-cells, brain, and adipose tissue and is localized in cell membrane and cytosol.

Function
SLC1A5 transports neutral amino acids coupled with sodium.
Interaction
Interacts with suppressyn and syncytin-1
Ligand/Receptor
Sodium ion and neutral amino acid
Cell Type
Erythrocytes, Glutamatergic Neurons
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Mitochondrial Function, Stem Cells
Molecular Family
Soluble Receptors
Antigen References
  1. Hassanein M, et al. 2013. Clin Cancer Res. 19:560–70.
  2. Canul-Tec J, et al. 2017. Nature. 544:446-51.
  3. Yoo HC, et al. 2020. Cell Metab. 31:267-83.
Gene ID
6510 View all products for this Gene ID
UniProt
View information about SLC1A5 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All SLC1A5 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-SLC1A5 W19125A WB,IHC-P,ICC,ICFC,IP
Go To Top Version: 1    Revision Date: 10/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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