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- Clone
- TA5F4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PALB, ATTR, Prealbumin, TBPA, TTR
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Congo red (A) and purified anti-Transthyretin, aggregated antibody (clone TA5F4) (B) staining of Transtheyretin amyloid deposits in formalin-fixed paraffin-embedded heart tissue of a patient with familial amyloidotic polyneuropathy (FAP). Following antigen retrieval using Antigen Retrieval Citra Plus (BioGenex), the tissue was incubated with the primary antibody at 3 µg/mL overnight at 4°C. Horse radish peroxidase conjugated anti-mouse IgG was used as the detection system. -
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Congo red (A) and purified anti-Transthyretin, aggregated antibody (clone TA5F4) (B) staining of Transtheyretin amyloid deposits in formalin-fixed paraffin-embedded heart tissue of a patient with familial amyloidotic polyneuropathy (FAP). Following antigen retrieval using Antigen Retrieval Citra Plus (BioGenex), the tissue was incubated with the primary antibody at 3 µg/mL overnight at 4°C. Horse radish peroxidase conjugated anti-mouse IgG was used as the detection system.-------
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 848101 | 25 µg | $112 | ||||
| 848102 | 100 µg | $288 | ||||
Transthyretin (TTR) is a carrier protein capable of transporting thyroid hormones in the plasma and cerebrospinal fluid, and retinol (vitamin A) in the plasma. TTR can form aberrant, insoluble amyloid fibrils that primarily deposit in a patient's heart, peripheral nerves, or both. The wild-type protein is associated with senile systemic amyloidosis (SSA) and is typically diagnosed in men over 70-years-old. Single or double mutations in TTR are primarily associated with familial amyloidotic polyneuropathy (FAP) or familial amyloid cardiopathy (FAC) which affects younger patients, often in their 30s. Clinical symptoms of TTR amyloidosis have historically been attributed to fibrils, but recent experimental evidence indicates that soluble TTR aggregates are also pathogenic.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A 1:1 mixture of wild-type Transthyretin fibrils and soluble aggregates of Y78F TTR.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
IHC-P - Verified
Direct ELISA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 2.0 µg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration of is 1.0 - 5.0 µg/ml is recommmended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone TA5F4 recognizes an epitope on aggregated transthyretin that encompasses mid- and N-terminal regions.
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Application References
(PubMed link indicates BioLegend citation) -
- Phay M, et al. 2014. Rejuvenation Res. 17(2):97. (Direct ELISA, Capture ELISA, IHC) PubMed
- RRID
-
AB_2632815 (BioLegend Cat. No. 848101)
AB_2632815 (BioLegend Cat. No. 848102)
Antigen Details
- Structure
- Transthyretin (TTR) is a β-sheet rich homotetrameric protein that has a molecular mass of 55 kD. Each monomer consists of 127 amino acids.
- Distribution
-
Primarily extracellular in blood and cerebrospinal fluid.
- Function
- The normal function of TTR is to transport thyroxine and retinol-binding protein in the blood and cerebrospinal fluid.
- Interaction
- Perlecan, receptor for advanced glycation end product (RAGE).
- Ligand/Receptor
- Thyroxine, retinol-binding protein bound to retinol.
- Cell Sources
- Liver, choroid plexus epithelium, retina pigment epithelium.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Carrier Proteins
- Antigen References
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1. Plante-Bordeneuve V, Said G. 2011. Lancet Neurol. 10:1086. PubMed
2. Pinney JH, et al. 2013. J. Am. Heart Assoc. 2:e000098. PubMed
3. Hawkins PN, et al. 2015. Ann Med. 47(8):625. PubMed - Gene ID
- 7276 View all products for this Gene ID
- UniProt
- View information about Transthyretin on UniProt.org
Other Formats
View All Transthyretin, aggregated Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Transthyretin, aggregated | TA5F4 | ELISA Capture,IHC-P,Direct ELISA |
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Purified anti-Transthyretin, aggregated
Congo red (A) and purified anti-Transthyretin, aggreg... 
Congo red (A) and purified anti-Transthyretin, aggreg... 
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