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Purified anti-TREM-2 Antibody

Pricing & Availability
Clone
6E9 (See other available formats)
Regulatory Status
RUO
Other Names
Triggering receptor expressed on myeloid cells 2, Trem, TREM-2, Trem2a, Trem2b, Trem2c, TREM2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
6E9_PURE_TREM-2_Antibody_030922
Thioglicollate-induced macrophages from the perithoneal cavity of a C57/BL6 mouse were stained purified anti-mouse TREM-2 (clone 6E9) (left) or purified rat IgG2b, κ isotype control (right) followed by PE anti-rat IgG and anti-mouse F4/80 APC. Previously to the staining the cell’s Fc receptors were blocked with purified anti-mouse CD16/32 recombinant (clone QA17A34).
  • 6E9_PURE_TREM-2_Antibody_030922
    Thioglicollate-induced macrophages from the perithoneal cavity of a C57/BL6 mouse were stained purified anti-mouse TREM-2 (clone 6E9) (left) or purified rat IgG2b, κ isotype control (right) followed by PE anti-rat IgG and anti-mouse F4/80 APC. Previously to the staining the cell’s Fc receptors were blocked with purified anti-mouse CD16/32 recombinant (clone QA17A34).
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824803 25 µg $129
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824804 100 µg $288
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Description

TREM-2 is a 26 kD transmembrane glycoprotein that consists of a single extracellular immunoglobulin-like domain, a transmembrane region with a charged lysine residue and a short cytoplasmic tail. It associates with DNAX-activation protein 12 (DAP12) for signaling and function. TREM-2 is expressed on immature monocyte-derived dendritic cells. After activation by microbial products or tumor necrosis factor (TNF) and TNF-related proteins, dendritic cells downregulate the expression of TREM-2. TREM-2 is also expressed by osteoclasts and microglia, where it is involved in bone modeling and brain function, respectively. Another role of TREM-2 might be promoting the removal of apoptotic cells, organic matrix and macromolecules by microglia.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse TREM-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house 
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Koga T, et al. 3004. Nature. 428:758. (FC, IP)
RRID
AB_2922646 (BioLegend Cat. No. 824803)
AB_2922646 (BioLegend Cat. No. 824804)

Antigen Details

Structure
26 kD transmembrane glycoprotein, member of the immunoglobulin superfamily
Distribution

Subset of macrophages, osteoclasts and microglia, immature dendritic cells

Function
Removal of apoptotic cells, organic matrix and macromolecules, bone modeling
Interaction
DNAX-activation protein 12 (DAP12)
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Antigen References
  1. Leyns CEG, et al. 2019. Nat Neurosci. 22:1217-22.
  2. Zhong L, et al. 2019. Nat Commun. 10:1365.
  3. Parhizkar S, et al. 2019. Nat Neurosci. 22:191-204.
  4. Filipello F, et al. 2018. Immunity. 48:979-91.
Gene ID
83433 View all products for this Gene ID
UniProt
View information about TREM-2 on UniProt.org
Go To Top Version: 1    Revision Date: 03/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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