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- Regulatory Status
- RUO
- Other Names
- E-cadherin, Cadherin-1, CAD1, CDH1, CADH1, ARC-1, CD324, CAM 120/80, Epithelial cadherin, Uvomorulin, CDHE, UVO
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Immobilized Recombinant Human E-Cadherin supports the adhesion of MCF-7 cells in a dose dependent manner. ED50 for this effect is 0.35 - 1.4 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells. -
Stability testing for Human E-Cadherin. Human E-Cadherin was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested for their ability to support the adhesion of MCF-7 cells.
E-cadherin, also known as cadherin-1, CDH1 and CD324, is a member of the cadherin superfamily. E-cadherin is widely expressed in epithelial cells. It is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region. E-cadherin functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. E-cadherin forms homophilic clusters via cis and trans interactions to stabilize catenin and the actin cytoskeleton in epithelial adherens junctions. E-cadherin and α-catenin can form the distinct complexes with β-catenin and plakoglobin, respectively. Mice lacking E-cadherin in the epidermis experience perinatal death and loss of barrier function. Regulating E-cadherin turnover is essential to modulate epithelial plasticity during tissue remodeling and stretching. In bacterial pathogenesis, the ectodomain of E-cadherin mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. E-cadherin binds to the αEβ7 integrin to mediate cell adhesion. It also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II as well as other extracellular proteins including the EGF receptor. E-cadherin is phosphorylated on multiple residues (S857, S866, S870, S872), and can be proteolytically cleaved at residue D769 by caspase-3. There are several diseases associated with dysregulation of E-cadherin, including Hailey-Hailey disease and Blepharocheilodontic syndrome 1 (BCDS1).
Product DetailsProduct Details
- Source
- Human E-Cadherin, amino acid Leu15 - Ile707 (Accession # P12830) with a C-terminal human IgG Fc tag, was expressed in 293E cells.
- Molecular Mass
- The 924 amino acid recombinant protein has a predicted molecular mass of approximately 102.5 kD. The DTT-reduced proteins migrate at approximately 85 – 115 kD, and non-reduced proteins migrate at approximately 190 - 230 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH7.2
- Endotoxin Level
- Less than 1.0 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.35 - 1.4 µg/mL as measured by the ability of immobilized protein to support the adhesion of MCF-7 cells. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Member of the cadherin superfamily
- Distribution
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Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells; plasma membrane.
- Function
- E-Cadherin is involved in development, bacterial pathogenesis, and tumor invasion.
- Interaction
- Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
- Ligand/Receptor
- αEβ7 integrin
- Bioactivity
- Measured by its ability to support the adhesion of MCF-7 cells.
- Cell Type
- Embryonic Stem Cells, Epithelial cells, Erythrocytes
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Adhesion Molecules
- Antigen References
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- Guillot C, et al. 2013. Science. 340: 1185.
- Yamada S, et al. 2005. Cell. 123: 889.
- Tunggal JA, et al. 2005. EMBI J. 24: 1146.
- Huber AH, et al. 2001. Cell. 105: 391.
- Nagafuchi A, et al. 1987. Nature. 329: 341.
- Cepek KL, et al. 1994. Nature. 372: 190.
- Butz S, et al. 1994. FEBS Letters. 355: 195.
- Fukunaga Y, et al. 2005. Cell Struct Funct. 30: 25.
- Gumbiner BM. 2005. Nat Rev Mol Cell Biol. 6: 622.
- Overduin M, et al. 1995. Science. 267:386.
- Boggon TJ, et al. 2002. Science. 296:1303.
- Berx G, et al. 1995. EMBO J. 14:6107.
- Perl AK, et al. 1998. Nature. 392:190.
- Ghoumid J, et al. 2017. Genet Med. 19: 1013.
- Hakuno M, et al. 2000. Br J Dermatol. 142: 702.
- Gene ID
- 999 View all products for this Gene ID
- UniProt
- View information about E-Cadherin on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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