Spark NIR™ 685 anti-human IFN-γ Antibody, IFN-γ, 4S.B3

Pricing & Availability

Clone: 4S.B3 (See other available formats)
Regulatory Status: RUO
Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype: Mouse IgG1, κ
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4S-B3_SPARKNIR685_IFN-gamma_Antibody_092520 — PMA+ ionomycin stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were fixed, permeabilized then stained with CD3 PE/Dazzle™ 594 and IFN-γ (clone 4S.B3) Spark NIR™ 685 (left) or mouse IgG1, κ Spark NIR™ 685 isotype control (right).

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502551

25 tests

$141

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502552

100 tests

$282

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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partially purified, native human IFN-γ
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: ELISA or ELISPOT Detection⁵: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry^3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization^1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
RRID: AB_2888716 (BioLegend Cat. No. 502551)
AB_2888716 (BioLegend Cat. No. 502552)

Antigen Details

Structure: Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources: CD8⁺ and CD4⁺ T cells, NK cells
Cell Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.
Regulation: Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID: 3458 View all products for this Gene ID
UniProt: View information about IFN-gamma on UniProt.org

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Related Protocols

Intracellular Flow Cytometry Staining Protocol

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Other Formats

View All IFN-γ Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human IFN-γ	4S.B3	ICFC
APC anti-human IFN-γ	4S.B3	ICFC
FITC anti-human IFN-γ	4S.B3	ICFC
Biotin anti-human IFN-γ	4S.B3	ELISA Detection,ELISPOT Detection,ICFC
Purified anti-human IFN-γ	4S.B3	ELISA,WB,ICFC
Alexa Fluor® 488 anti-human IFN-γ	4S.B3	ICFC
Alexa Fluor® 647 anti-human IFN-γ	4S.B3	ICFC
Alexa Fluor® 700 anti-human IFN-γ	4S.B3	ICFC
Pacific Blue™ anti-human IFN-γ	4S.B3	ICFC
PerCP/Cyanine5.5 anti-human IFN-γ	4S.B3	ICFC
APC/Cyanine7 anti-human IFN-γ	4S.B3	ICFC
PE/Cyanine7 anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 421™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 570™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 605™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 650™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 711™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 785™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 510™ anti-human IFN-γ	4S.B3	ICFC
PE/Dazzle™ 594 anti-human IFN-γ	4S.B3	ICFC
APC/Fire™ 750 anti-human IFN-γ	4S.B3	ICFC
PerCP anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 750™ anti-human IFN-γ	4S.B3	ICFC
KIRAVIA Blue 520™ anti-human IFN-γ Antibody	4S.B3	ICFC
Spark NIR™ 685 anti-human IFN-γ Antibody	4S.B3	ICFC
APC anti-human IFN-γ	4S.B3	ICFC
Spark Blue™ 515 anti-human IFN-γ	4S.B3	ICFC
Spark UV™ 387 anti-human IFN-γ	4S.B3	ICFC
Spark PLUS UV395™ anti-human IFN-γ	4S.B3	ICFC
PE anti-human IFN-γ	4S.B3	ICFC
GMP APC anti-human IFN-γ	4S.B3	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 09/25/2020

For Research Use Only. Not for diagnostic or therapeutic use.

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