Spark NIR™ 685 anti-mouse CD45 Antibody, CD45, 30-F11

Pricing & Availability

Clone: 30-F11 (See other available formats)
Regulatory Status: RUO
Other Names: T200, Ly-5, LCA
Isotype: Rat IgG2b, κ
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Product Citations: publications

C57BL/6 mouse splenocytes were stained with CD45 (clone 30-F11) Spark NIR™ 685 (filled histogram.) Open histogram represents unstained cells.

Compare all formats See Spark NIR™ 685 spectral data

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103167

25 µg

$136

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103168

100 µg

$364

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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation³, complement-dependent cytotoxicity^1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)^4,6, Western blotting⁷, and spatial biology (IBEX)^10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
RRID: AB_2819792 (BioLegend Cat. No. 103167)
AB_2819792 (BioLegend Cat. No. 103168)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type: B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
APC anti-mouse CD45	30-F11	FC
Biotin anti-mouse CD45	30-F11	FC
FITC anti-mouse CD45	30-F11	FC
PE anti-mouse CD45	30-F11	FC
PE/Cyanine5 anti-mouse CD45	30-F11	FC
Purified anti-mouse CD45	30-F11	FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45	30-F11	FC
APC/Cyanine7 anti-mouse CD45	30-F11	FC
Alexa Fluor® 488 anti-mouse CD45	30-F11	FC,SB
Alexa Fluor® 647 anti-mouse CD45	30-F11	FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45	30-F11	FC
Alexa Fluor® 700 anti-mouse CD45	30-F11	FC,SB
PerCP/Cyanine5.5 anti-mouse CD45	30-F11	FC
PerCP anti-mouse CD45	30-F11	FC
Alexa Fluor® 594 anti-mouse CD45	30-F11	IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45	30-F11	FC,SB
Brilliant Violet 570™ anti-mouse CD45	30-F11	FC
Brilliant Violet 510™ anti-mouse CD45	30-F11	FC
Brilliant Violet 605™ anti-mouse CD45	30-F11	FC
Purified anti-mouse CD45 (Maxpar® Ready)	30-F11	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45	30-F11	FC
Brilliant Violet 711™ anti-mouse CD45	30-F11	FC
Brilliant Violet 785™ anti-mouse CD45	30-F11	FC
Brilliant Violet 650™ anti-mouse CD45	30-F11	FC
APC/Fire™ 750 anti-mouse CD45	30-F11	FC
Brilliant Violet 750™ anti-mouse CD45	30-F11	FC
TotalSeq™-A0096 anti-mouse CD45	30-F11	PG
TotalSeq™-B0096 anti-mouse CD45	30-F11	PG
Ultra-LEAF™ Purified anti-mouse CD45	30-F11	FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45	30-F11	FC
Spark NIR™ 685 anti-mouse CD45	30-F11	FC
TotalSeq™-C0096 anti-mouse CD45	30-F11	PG
Spark YG™ 570 anti-mouse CD45	30-F11	IHC-F
PE/Fire™ 640 anti-mouse CD45	30-F11	FC
APC/Fire™ 810 anti-mouse CD45	30-F11	FC
PE/Fire™ 700 anti-mouse CD45	30-F11	FC
Spark Violet™ 538 anti-mouse CD45	30-F11	FC
Spark YG™ 593 anti-mouse CD45	30-F11	FC
Spark Blue™ 574 anti-mouse CD45 Antibody	30-F11	FC
Spark Blue™ 515 anti-mouse CD45	30-F11	FC
Spark UV™ 387 anti-mouse CD45	30-F11	FC
PE/Fire™ 810 anti-mouse CD45	30-F11	FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™)	30-F11	FC
Spark PLUS UV395™ anti-mouse CD45	30-F11	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/07/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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