- Clone
- MHM-88 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin M
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.
Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended. - Additional Product Notes
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For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
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Application References
(PubMed link indicates BioLegend citation) -
- Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Antigen Details
- Structure
- Ig family
- Distribution
-
B cells
- Function
- B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 3507 View all products for this Gene ID
- UniProt
- View information about IgM on UniProt.org
Related FAQs
- What are Flexi-Fluors?
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Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
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We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
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Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
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We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
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Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
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Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
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Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All IgM Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IgM
Over night cultured human peripheral blood lymphocytes stain... -
PE anti-human IgM
Human peripheral blood lymphocytes were stained with CD19 FI... -
Biotin anti-human IgM
Human peripheral blood lymphocytes stained with CD19 APC and... -
FITC anti-human IgM
Human peripheral blood lymphocytes stained with MHM-88 FITC -
APC anti-human IgM
Human peripheral blood lymphocytes stained with MHM-88 APC -
PerCP/Cyanine5.5 anti-human IgM
Overnight cultured human peripheral blood lymphocytes were s... -
Pacific Blue™ anti-human IgM
Over night cultured human peripheral blood lymphocytes stain... -
Brilliant Violet 421™ anti-human IgM
-
APC/Cyanine7 anti-human IgM
Human peripheral blood lymphocytes were cultured overnight a... -
Brilliant Violet 570™ anti-human IgM
Isolated human peripheral blood mononuclear cells were stain... -
Brilliant Violet 510™ anti-human IgM
Isolated human peripheral blood mononuclear cells were stain... -
Brilliant Violet 605™ anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ... -
Brilliant Violet 650™ anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ... -
Purified anti-human IgM (Maxpar® Ready)
Human PBMCs stained with 142Nd-anti-CD19 (HIB19) and 172Yb-a... -
PE/Dazzle™ 594 anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ... -
PE/Cyanine7 anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ... -
Alexa Fluor® 488 anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ... -
Alexa Fluor® 647 anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ... -
Alexa Fluor® 700 anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ... -
Brilliant Violet 711™ anti-human IgM
Isolated human peripheral blood mononuclear cells were stain... -
TotalSeq™-A0136 anti-human IgM
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TotalSeq™-C0136 anti-human IgM
-
Brilliant Violet 785™ anti-human IgM
Human peripheral blood lymphocytes were cultured overnight a... -
APC/Fire™ 750 anti-human IgM
Human peripheral blood lymphocytes were cultured overnight a... -
TotalSeq™-B0136 anti-human IgM
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Spark Violet™ 423 anti-human IgM Antibody
Overnight cultured human peripheral blood lymphocytes were s... -
TotalSeq™-D0136 anti-human IgM
-
Spark Blue™ 550 anti-human IgM
Human peripheral blood lymphocytes were cultured overnight a... -
Spark Blue™ 515 anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ... -
PE/Fire™ 700 anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ... -
Spark YG™ 593 anti-human IgM
Overnight cultured human peripheral blood lymphocytes staine... -
PE/Fire™ 640 anti-human IgM
Human peripheral blood mononuclear cells were stained with a... -
Spark Red™ 718 anti-human IgM (Flexi-Fluor™)
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APC anti-human IgM
Typical results from overnight cultured human peripheral blo... -
PE anti-human IgM
Typical results from overnight cultured human peripheral blo... -
Brilliant Violet 750™ anti-human IgM
Overnight cultured human peripheral blood mononuclear cells ...
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