- Clone
- M5E2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- III 329
- Other Names
- LPS receptor
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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301861 | 100 µg | $237 | ||||
301862 | 1 mg | $688 |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
-
Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
- Williams KC, et al. 2001. J. Exp. Med. 193:905.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
- Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- RRID
-
AB_2814124 (BioLegend Cat. No. 301861)
AB_2814124 (BioLegend Cat. No. 301862)
Antigen Details
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434. - Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD14 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD14
Human peripheral blood monocytes stained with M5E2 APC -
FITC anti-human CD14
Human peripheral blood monocytes stained with M5E2 FITC -
PE anti-human CD14
Human peripheral blood monocytes stained with M5E2 PE -
Purified anti-human CD14
Human peripheral blood monocytes stained with M5E2 APC -
PE/Cyanine7 anti-human CD14
Human peripheral blood monocytes stained with M5E2 PE/Cyanin... -
Alexa Fluor® 488 anti-human CD14
Human peripheral blood monocytes stained with M5E2 Alexa Flu... -
Alexa Fluor® 647 anti-human CD14
Human peripheral blood monocytes stained with M5E2 Alexa Flu... -
Ultra-LEAF™ Purified anti-human CD14
Human peripheral blood monocytes were stained with Ultra-LEA... -
Pacific Blue™ anti-human CD14
Human peripheral blood monocytes stained with clone M5E2 Pac... -
APC/Cyanine7 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Alexa Fluor® 700 anti-human CD14
Human peripheral blood monocytes stained with M5E2 Alexa Flu... -
PerCP/Cyanine5.5 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Biotin anti-human CD14
Human peripheral blood monocytes stained with biotinylated M... -
Brilliant Violet 421™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 570™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 605™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 650™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 711™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 785™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Brilliant Violet 510™ anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Purified anti-human CD14 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 160Gd-an... -
PerCP anti-human CD14
Human peripheral blood monocytes were stained with PerCP CD1... -
FITC anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PE/Dazzle™ 594 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
Pacific Blue™ anti-human CD14
Typical results from human peripheral blood monocytes staine... -
APC/Fire™ 750 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
APC anti-human CD14
Typical results from human peripheral blood monocytes staine... -
TotalSeq™-A0081 anti-human CD14
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TotalSeq™-B0081 anti-human CD14
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TotalSeq™-C0081 anti-human CD14
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PE anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PE/Cyanine5 anti-human CD14
Human peripheral blood monocytes were stained with CD14 (clo... -
TotalSeq™-D0081 anti-human CD14
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APC/Fire™ 750 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP FITC anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PE/Cyanine7 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP APC anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP PE anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PE/Dazzle™ 594 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP Pacific Blue™ anti-human CD14
Typical results from human peripheral blood monocytes staine... -
GMP APC/Fire™ 750 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
PerCP/Cyanine5.5 anti-human CD14
Figure Legend: Typical results from human peripheral blood m... -
Spark Violet™ 500 anti-human CD14
Human peripheral blood monocytes were stained with anti-huma... -
GMP PE/Dazzle™ 594 anti-human CD14
Typical results from human peripheral blood monocytes staine... -
APC/Fire™ 810 anti-human CD14
Human peripheral blood monocytes were stained with anti-huma... -
PE/Fire™ 700 anti-human CD14
Human peripheral blood monocytes were stained with anti-huma...
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