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Ultra-LEAF™ Purified anti-human CD154 Antibody

Pricing & Availability
Clone
24-31 (See other available formats)
Regulatory Status
RUO
Other Names
CD40L, gp39, TRAP, T-BAM, TNFSF5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
24-31
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with CD154 (clone 24-31) PE
  • 24-31
    PMA+ionomycin-stimulated human PBMCs (6 hours) stained with CD154 (clone 24-31) PE
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310827 100 µg $226
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310828 1 mg $655
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3 and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF)
  2. Rushworth SA, et al. 2002. Transplantation 73:635. (Block)
  3. Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF)
  4. Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block)
  5. Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed
  6. Kuchen S, et al. 2007. J. Immunol. 179:5886.
  7. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
RRID
AB_11150400 (BioLegend Cat. No. 310827)
AB_11150400 (BioLegend Cat. No. 310828)

Antigen Details

Structure
TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution

Activated T cells

Function
B cell costimulation
Ligand/Receptor
CD40
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037.

Gene ID
959 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD154 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD154 24-31 FC,IHC-F
FITC anti-human CD154 24-31 FC,IHC-F
PE anti-human CD154 24-31 FC
PE/Cyanine5 anti-human CD154 24-31 FC
APC anti-human CD154 24-31 FC
Biotin anti-human CD154 24-31 FC,IHC-F
Alexa Fluor® 488 anti-human CD154 24-31 FC,IHC-F
Alexa Fluor® 647 anti-human CD154 24-31 FC,IHC-F
Pacific Blue™ anti-human CD154 24-31 FC
Brilliant Violet 785™ anti-human CD154 24-31 FC
APC/Cyanine7 anti-human CD154 24-31 FC
Brilliant Violet 421™ anti-human CD154 24-31 FC,IHC-F
Brilliant Violet 605™ anti-human CD154 24-31 FC
Ultra-LEAF™ Purified anti-human CD154 24-31 FC,Block,IHC-F
Brilliant Violet 510™ anti-human CD154 24-31 FC,IHC-F
PE/Cyanine7 anti-human CD154 24-31 FC
PerCP/Cyanine5.5 anti-human CD154 24-31 FC
Brilliant Violet 711™ anti-human CD154 24-31 FC
Purified anti-human CD154 (Maxpar® Ready) 24-31 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD154 24-31 FC
TotalSeq™-A0032 anti-human CD154 24-31 PG
Alexa Fluor® 700 anti-human CD154 24-31 FC
APC/Fire™ 750 anti-human CD154 24-31 FC
TotalSeq™-C0032 anti-human CD154 24-31 PG
TotalSeq™-B0032 anti-human CD154 24-31 PG
TotalSeq™-D0032 anti-human CD154 24-31 PG
PE/Fire™ 640 anti-human CD154 24-31 FC
PE/Fire™ 810 anti-human CD154 24-31 FC
PerCP/Fire™ 780 anti-human CD154 24-31 FC
PE/Fire™ 700 anti-human CD154 24-31 FC
Spark Red™ 718 anti-human CD154 (Flexi-Fluor™) 24-31 FC
Brilliant Violet 650™ anti-human CD154 24-31 FC
Spark Blue™ 574 anti-human CD154 (Flexi-Fluor™) 24-31 FC
Spark Blue™ 550 anti-human CD154 (Flexi-Fluor™) 24-31 FC
PE/Cyanine7 anti-human CD154 24-31 FC
Go To Top Version: 2    Revision Date: 01/13/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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