Alexa Fluor® 488 anti-ATG5 Antibody

Pricing & Availability
Clone
177.19 (See other available formats)
Regulatory Status
RUO
Other Names
Autophagy protein 5
Isotype
Mouse IgG1, κ
177dot19_A488_ATG5_Antibody_101018
ICC staining of Alexa Fluor® 488 anti-ATG5 antibody (clone 177.19) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • 177dot19_A488_ATG5_Antibody_101018
    ICC staining of Alexa Fluor® 488 anti-ATG5 antibody (clone 177.19) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 488 spectral data
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847407 25 µg $159.00
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847408 100 µg $335.00
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Description

Autophagy protein 5 (ATG5) is involved in autophagic vesicle formation. ATG5 forms a complex with autophagy protein 12 (ATG12), which has E3 ubiquitin ligase activity, and is associated with autophagosome vesicle formation. ATG5 detaches from membranes immediately before or after autophagosome formation. ATG5 is also involved in mitochondrial quality control after oxidative damage, the innate anti-viral immune response, and apoptosis.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
ATG5 peptide conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Reactivity to Drosophila was only verified with the purified format.

RRID
AB_2783417 (BioLegend Cat. No. 847407)
AB_2783417 (BioLegend Cat. No. 847408)

Antigen Details

Structure
Human ATG5 is a 275 amino acid protein with a molecular mass of 32 kD.
Distribution

Tissue distribution: Ubiquitous.
Cellular distribution: Vacuole, lysosome, and to a lesser extent mitochondria.

Function
ATG5 is involved in many cellular processes including autophagic vesicle formation, mitochondrial quality control after oxidative damage, innate anti-viral immune response, and apoptosis.
Interaction
ATG12, LC3B, ATG16, TECPR1, Autophagy-linked FYVE protein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References

1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed
2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed
3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed

Gene ID
9474 View all products for this Gene ID
UniProt
View information about ATG5 on UniProt.org
Go To Top Version: 1    Revision Date: 10/11/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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