Alexa Fluor® 488 anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
30-F11_Alx488_090507
C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 488
  • 30-F11_Alx488_090507
    C57BL/6 mouse splenocytes stained with 30-F11 Alexa Fluor® 488
  • 33_Mouse_Spleen_CD169_CD45
    Confocal image of C57BL/6 mouse spleen sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD169 (green) in Cycle 2 and CD45 (blue) in Cycle 3. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 488 spectral data
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103121 25 µg $101.00
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103122 100 µg $229.00
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Preda MB, et al. 2021. Cell Death Dis. 12:566. PubMed
  2. Kuo PC, et al. 2021. Brain Commun. 3:fcab187. PubMed
  3. Wu Y, et al. 2021. Immunity. 54:2595. PubMed
  4. Wemlinger SM, et al. 2022. J Immunol. 208:1566. PubMed
  5. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  6. Frederico B, et al. 2022. Dev Cell. 57:1957. PubMed
  7. Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed
  8. Olivares-González L, et al. 2022. Antioxidants (Basel). 12: . PubMed
  9. Gilman KE, et al. 2023. Front Immunol. 13:1045710. PubMed
  10. Parida PK, et al. 2022. Cell Metab. 34:90. PubMed
  11. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  12. Luff SA, et al. 2022. Nat Cell Biol. 24:616. PubMed
  13. Battis K, et al. 2022. J Neurosci. 42:7673. PubMed
  14. Abdel-Haq R, et al. 2022. Elife. 11:. PubMed
  15. Axelrod ML, et al. 2022. Nature. 611:818. PubMed
  16. Gómez-Salinero JM, et al. 2022. Nat Cardiovasc Res. 1:882. PubMed
  17. Fan Z, et al. 2023. Angiogenesis. . PubMed
  18. Gilman KE, et al. 2023. Cancers (Basel). 15:. PubMed
  19. Palakurthi B, et al. 2023. Nat Commun. 14:2109. PubMed
  20. Fixsen BR, et al. 2023. Nat Immunol. . PubMed
  21. Marko C, et al. 2014. Invest Ophthalmol Vis Sci . 55:291. PubMed
  22. Giorgetti E, et al. 2020. Cell Reports. 29(6):1539-1554.e7.. PubMed
  23. Kazakevych J, et al. 2020. Genome Biol. 21:64. PubMed
  24. Sakai M, et al. 2020. Immunity. 51(4):655-670. PubMed
  25. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  26. Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
  27. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  28. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  29. Khandelwal P, et al. 2013. PLoS One. 8:64193. PubMed
  30. Masschelein E, et al. 2020. Skelet Muscle. 10:21. PubMed
  31. Martínez-Sabadell A, et al. 2022. STAR Protoc. 3:101712. PubMed
  32. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  33. Bornes L, et al. 2021. Life Sci Alliance. 4:00. PubMed
  34. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  35. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  36. DeDreu J, et al. 2020. FASEB J. 34:9316. PubMed
  37. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  38. Hallett JM, et al. 2022. Cell Stem Cell. 29:355. PubMed
  39. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  40. Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed
  41. Chen I, et al. 2016. Sci Rep. 6:27195. PubMed
  42. Duarte J, et al. 2016. J Immunol. 197(12):4838-4847. PubMed
  43. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
  44. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  45. Gutknecht M, et al. 2017. Nat Commun. 10.1038/s41467-017-00488-6. PubMed
  46. Ferrari G, et al. 2011. Invest Ophthalmol Vis Sci. 52:2532. PubMed
  47. Mizbani A, et al. 2016. Development. 143: 4137 - 4148. PubMed
  48. Burns JC, et al. 2020. eLife. 9:00. PubMed
  49. Wara AK, et al. 2020. Cell Rep. 33:108550. PubMed
  50. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  51. Lustgarten Guahmich N, et al. 2020. Angiogenesis. 23:443. PubMed
  52. Limbad C, et al. 2022. iScience. 25:103848. PubMed
  53. Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
  54. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  55. Dobson HE, et al. 2020. Mucosal Immunol. 0.901388889. PubMed
  56. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  57. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  58. Shibad V, et al. 2021. Front Immunol. 12:722451. PubMed
  59. Wüthrich M, et al. 2021. MBio. 12:e0201821. PubMed
  60. Li D, et al. 2022. Commun Biol. 5:271. PubMed
  61. Braun D, et al. 2013. J Biol Chem. 288:2689. PubMed
  62. Pang M, et al. 2009. J Immunol. 182:7001. PubMed
  63. Wu X, et al. 2018. Cell. 172:423. PubMed
  64. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  65. Ding H, et al. 2016. Nat Commun. 7:11533. PubMed
  66. Franklin DA, et al. 2020. JCI Insight. 5:. PubMed
  67. Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed
  68. Kazakevych J, et al. 2019. Sci Rep. 9:10410. PubMed
  69. Peng W, et al. 2018. Mol Cell Biol. 38:e00427. PubMed
  70. Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed
  71. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
  72. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  73. Pantelidou C, et al. 2022. NPJ Breast Cancer. 8:102. PubMed
  74. Lasch M, et al. 2020. Front Physiol. 11:576736. PubMed
  75. DeDreu J, et al. 2022. FASEB J. 36:e21995. PubMed
  76. Li Q, et al. 2020. Am J Pathol. 190:2453. PubMed
  77. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  78. Wang Y, et al. 2019. Front Cell Infect Microbiol. 9:286. PubMed
  79. Ye Y, et al. 2011. Am J Physiol Lung Cell Mol Physiol. 300:L216. PubMed
  80. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  81. Tian F, et al. 2016. Nat Commun. 7:13283. PubMed
  82. Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
  83. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  84. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  85. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  86. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
RRID
AB_493531 (BioLegend Cat. No. 103121)
AB_493531 (BioLegend Cat. No. 103122)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 (Flexi-Fluor™) 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Go To Top Version: 5    Revision Date: 04/20/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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