Alexa Fluor® 488 anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
RMM-1_AF488_IgM_Antibody_FC_1_111913
C57BL/6 splenocytes were stained with CD19 APC and anti-IgM (clone RMM-1) Alexa Fluor® 488 (top) or rat IgG2a Alexa Fluor® 488 isotype control (bottom).
  • RMM-1_AF488_IgM_Antibody_FC_1_111913
    C57BL/6 splenocytes were stained with CD19 APC and anti-IgM (clone RMM-1) Alexa Fluor® 488 (top) or rat IgG2a Alexa Fluor® 488 isotype control (bottom).
  • RMM-1_AF488_IgM_Antibody_FC_2_111913
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability
406521 25 µg $89.00
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406522 100 µg $229.00
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Melgrati S, et al. 2023. PLoS Biol. e3002111:21. PubMed
  2. Massenet-Regad L, et al. 2023. iScience. 108367:26. PubMed
  3. Yang K, et al. 2018. J Exp Med. 215:2600. PubMed
  4. Vrijens P, et al. 2019. J Gen Virol. 100:583. PubMed
RRID
AB_2562858 (BioLegend Cat. No. 406521)
AB_2562858 (BioLegend Cat. No. 406522)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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