FITC anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
RMM-1_FITC_042909.jpg
C57BL/6 splenocytes stained with RMM-1 FITC
  • RMM-1_FITC_042909.jpg
    C57BL/6 splenocytes stained with RMM-1 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
406505 50 µg $83.00
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406506 500 µg $200.00
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
  2. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  3. Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed
  4. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  5. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
  6. Vijayan K, et al. 2021. Cell Reports. 36(5):109489. PubMed
  7. Reed S, et al. 2014. PLoS One. 9:112126. PubMed
  8. Tang H, et al. 2022. iScience. 25:104209. PubMed
  9. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  10. Wilder BK, et al. 2022. NPJ Vaccines. 7:58. PubMed
  11. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  12. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  13. Hutter K, et al. 2020. FEBS J. . PubMed
  14. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  15. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  16. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  17. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  18. Jelinek D, et al. 2014. J Immunol. 192:3548. PubMed
  19. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  20. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  21. Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
  22. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  23. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  24. Carbonetti S et al. 2017. Journal of immunological methods. 448:66-73 . PubMed
  25. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
RRID
AB_315055 (BioLegend Cat. No. 406505)
AB_315055 (BioLegend Cat. No. 406506)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org
Go To Top Version: 2    Revision Date: 01/29/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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