Brilliant Violet 510™ anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
RMM-1_BV510_IgM_Antibody_1_013117
C57BL/6 splenocytes were stained with CD19 PE and anti-mouse IgM (clone RMM-1) Brilliant Violet 510™ (top) or rat IgG2a, κ Brilliant Violet 510™ isotype control (bottom).
  • RMM-1_BV510_IgM_Antibody_1_013117
    C57BL/6 splenocytes were stained with CD19 PE and anti-mouse IgM (clone RMM-1) Brilliant Violet 510™ (top) or rat IgG2a, κ Brilliant Violet 510™ isotype control (bottom).
  • RMM-1_BV510_IgM_Antibody_2_013117
Compare all formats See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Check Availability
406531 50 µg $264.00
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  2. DiPiazza AT, et al. 2021. Immunity. 54(8):1869-1882.e6. PubMed
  3. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  4. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  5. Nascimento DC, et al. 2021. Immunity. :. PubMed
RRID
AB_2650758 (BioLegend Cat. No. 406531)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org
Go To Top Version: 1    Revision Date: 02/01/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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