Alexa Fluor® 488 anti-Nestin Antibody

Pricing & Availability
Clone
10C2 (See other available formats)
Regulatory Status
RUO
Other Names
NES
Isotype
Mouse IgG1, κ
10C2_A488_Nestin_Antibody_1_061919
HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum for more than one hour at room temperature. Then the cells were stained with 5 µg/ml of anti-Nestin (clone 10C2) Alexa Fluor® 488 overnight, followed by Flash Phalloidin™ Red 594 (red) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (purple). The image was captured with a 40X objective.
  • 10C2_A488_Nestin_Antibody_1_061919
    HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum for more than one hour at room temperature. Then the cells were stained with 5 µg/ml of anti-Nestin (clone 10C2) Alexa Fluor® 488 overnight, followed by Flash Phalloidin™ Red 594 (red) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (purple). The image was captured with a 40X objective.
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability
656812 100 µg $358.00
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Description

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1.

Application References

(PubMed link indicates BioLegend citation)
  1. Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
Product Citations
  1. Schober S, et al. 2023. Cancer Immunol Immunother. 3621:72. PubMed
RRID
AB_2629793 (BioLegend Cat. No. 656812)

Antigen Details

Structure
1621 amino acids with predicted molecular weight of 177 kD. The observed molecular weight is larger than 250 kD.
Distribution

Cytosol

Function
Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction
Vimentin, alpha-internexin and FHOD3
Cell Type
Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Intermediate Filaments
Antigen References

1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.

Gene ID
10763 View all products for this Gene ID
UniProt
View information about Nestin on UniProt.org
Go To Top Version: 1    Revision Date: 09/30/2016

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