Alexa Fluor® 647 anti-Nestin Antibody

Pricing & Availability
Clone
10C2 (See other available formats)
Regulatory Status
RUO
Other Names
NES
Isotype
Mouse IgG1, κ
1_10C2_AF647_Nestin_Antibody_IF_042116
HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum (FBS) for 30 minutes at room temperature. Then the cells were stained with 5 µg/ml of anti-Nestin (clone 10C2) Alexa Fluor® 647 (red) overnight, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 20X objective.
  • 1_10C2_AF647_Nestin_Antibody_IF_042116
    HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum (FBS) for 30 minutes at room temperature. Then the cells were stained with 5 µg/ml of anti-Nestin (clone 10C2) Alexa Fluor® 647 (red) overnight, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 20X objective.
  • 2_10C2_A647_Nestin_Antibody_IHC-P_122917
    Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate-Buffered 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Tween 20 in PBS and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Alexa Fluor® 594 anti-human Cadherin 11 (clone 16G5) antibody (green) and Alexa Fluro®647 anti-Nestin (clone 10C2) antibody (red) overnight at 4°C. Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
656810 100 µg $347.00
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Description

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1.

Application References

(PubMed link indicates BioLegend citation)
  1. Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
Product Citations
  1. Woloshuk A, et al. 2021. Cytometry A. 99:707. PubMed
RRID
AB_2616772 (BioLegend Cat. No. 656810)

Antigen Details

Structure
1621 amino acids with predicted molecular weight of 200-220 kD
Distribution

Cytosol

Function
Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction
Vimentin, alpha-internexin and FHOD3
Cell Type
Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Intermediate Filaments
Antigen References

1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.

Gene ID
10763 View all products for this Gene ID
UniProt
View information about Nestin on UniProt.org
Go To Top Version: 1    Revision Date: 04/21/2016

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