- Clone
- 10C2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NES
- Isotype
- Mouse IgG1, κ
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HUVEC cells were treated with the True-Nuclear™ Transcription Factor Buffer Set, then stained with Nestin (clone 10C2) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 656805 | 25 tests | $153.00 | |||
| 656806 | 100 tests | $335.00 | |||
Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
-
Application References
(PubMed link indicates BioLegend citation) -
- Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
- Product Citations
-
- RRID
-
AB_2566381 (BioLegend Cat. No. 656805)
AB_2566381 (BioLegend Cat. No. 656806)
Antigen Details
- Structure
- 1621 amino acids with predicted molecular weight of 200-220 kD
- Distribution
-
Cytosol
- Function
- Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
- Interaction
- Vimentin, alpha-internexin and FHOD3
- Cell Type
- Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Intermediate Filaments
- Antigen References
-
1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423. - Gene ID
- 10763 View all products for this Gene ID
- UniProt
- View information about Nestin on UniProt.org
Other Formats
View All Nestin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Nestin | 10C2 | WB,ICC,IHC-P |
| Alexa Fluor® 594 anti-Nestin | 10C2 | ICC,IHC-P |
| Brilliant Violet 421™ anti-Nestin | 10C2 | ICC,IHC-P |
| PE anti-Nestin | 10C2 | ICFC |
| Alexa Fluor® 647 anti-Nestin | 10C2 | ICC,IHC-P |
| Alexa Fluor® 488 anti-Nestin | 10C2 | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Nestin
NTERA-2 cells were fixed with 2% paraformaldehyde (PFA) for ... 
NTERA-2 cells were fixed with 2% paraformaldehyde (PFA) for ... 
Total lysates (15 µg protein) from HeLa (Low expression nega... -
Alexa Fluor® 594 anti-Nestin
HUVEC cells were fixed with 1% paraformaldehyde (PFA), perme... 
Human paraffin-embedded kidney tissue slices were prepared w... -
Brilliant Violet 421™ anti-Nestin
HUVEC cells were fixed with 1% paraformaldehyde (PFA), perme... 
Human paraffin-embedded ovary tissue slices were prepared wi... -
PE anti-Nestin
HUVEC cells were treated with the True-Nuclear™ Transcriptio... -
Alexa Fluor® 647 anti-Nestin
HUVEC cells were fixed with 1% paraformaldehyde (PFA), perme... 
Human paraffin-embedded kidney tissue slices were prepared w... -
Alexa Fluor® 488 anti-Nestin
HUVEC cells were fixed with 1% paraformaldehyde (PFA), perme...