- Clone
- Poly4053 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Goat Polyclonal IgG
-
Frozen mouse intestine stained with purified anti-Ankyrin-B (green), E-Cadherin (red) and DAPI (blue) followed by anti-goat Alexa Fluor® 488 and anti-rabbit Alexa Fluor® 568. Image generously submitted to the 2017 Cell Life Imaging Competition by Kathleen Ignatoski from University of Michigan.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405319 | 100 µg | $183.00 | |||
This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
-
Application References
(PubMed link indicates BioLegend citation) -
- Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
- Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
- Winters T, et al. 2014. EMBO J. 33:1256-70.
- Product Citations
-
- RRID
-
AB_2563044 (BioLegend Cat. No. 405319)
Antigen Details
- Distribution
-
B cells
- Gene ID
- 16059 View all products for this Gene ID
- UniProt
- View information about IgG on UniProt.org
Other Formats
View All IgG Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Goat anti-mouse IgG (minimal x-reactivity)
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Biotin Goat anti-mouse IgG (minimal x-reactivity)
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FITC Goat anti-mouse IgG (minimal x-reactivity)
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HRP Goat anti-mouse IgG (minimal x-reactivity)
-
PE Goat anti-mouse IgG (minimal x-reactivity)
-
Purified Goat anti-mouse IgG (minimal x-reactivity)
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Cyanine3 Goat anti-mouse IgG (minimal x-reactivity)
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PE/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes stained with purified mou... -
PerCP/Cyanine5.5 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes stained with purified mou... -
DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity)
Hela cells were stained with anti-Tubulin-γ and secondarily ... -
DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity)
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Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity)
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ... 
Frozen human tonsil section was fixed with 4% paraformaldehy... 
Human paraffin-embedded placenta tissue slices were prepared... -
APC/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes stained with purified mou... -
Brilliant Violet 421™ Goat anti-mouse IgG (minimal x-reactivity)
293E cells were stained with purified anti-tubulin-alpha (cl... 
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity)
Frozen mouse intestine stained with purified anti-Ankyrin-B ... -
Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity)
Human paraffin-embedded tonsil tissue slices were prepared w... -
Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity)
HUVEC cells were fixed with 1% paraformaldehyde (PFA), and t... -
Brilliant Violet 605™ Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes were stained with purifie... -
Brilliant Violet 510™ Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes were stained with purifie... -
PE/Dazzle™ 594 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes were stained with purifie... -
PerCP Goat anti-mouse IgG (minimal x-reactivity)
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APC/Fire™ 750 Goat anti-mouse IgG (minimal x-reactivity)
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Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes stained with purified ant... 
Human paraffin-embedded tonsil tissue slices were prepared w... -
Spark Red™ 718 Goat anti-mouse IgG (minimal x-reactivity)
IHC staining of Spark Red™ 718 Goat anti-mouse IgG (clone Po... 
IHC staining of Spark Red™ 718 Goat anti-mouse IgG (clone Po... 
HeLa cells were fixed with Fixation Buffer (Cat. No. 420801)...