Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Poly4053_A594_IgG_Antibody_1_102820
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml of purified anti-human EGFR (clone AY13), followed by 2.5 µg/ml of Alexa Fluor® 594 anti-mouse IgG (clone Poly4053) (red). Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
  • Poly4053_A594_IgG_Antibody_1_102820
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml of purified anti-human EGFR (clone AY13), followed by 2.5 µg/ml of Alexa Fluor® 594 anti-mouse IgG (clone Poly4053) (red). Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
  • Poly4053_A594_IgG_Antibody_2_102820
    Frozen human tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-human CD19 (clone SJ25C1) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • Poly4053_A594_IgG_Antibody_3_102820
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD309 (clone A16085I) at 4°C overnight, followed by 2.5 µg/ml of Alexa Fluor® 594 anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Compare all formats See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Check Availability
405326 100 µg $112.00
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-F, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations
  1. Ambade AS, et al. 2019. Transl Res. 203:15. PubMed
  2. Farrell K, et al. 2022. Cell Rep. 41:111470. PubMed
  3. Aljobaily N, et al. 2022. Nutrients. 15: . PubMed
  4. Gurusamy M, et al. 2023. Eur J Med Res. 28:71. PubMed
  5. Beaver JT, et al. 2020. Hum Vaccin Immunother. 2.119444444. PubMed
  6. Tada T, et al. 2022. Cell Rep. 38:110237. PubMed
  7. Beaver JT, et al. 2020. Hum Vaccin Immunother. 2.105555556. PubMed
  8. Gao X et al. 2018. Stem cell research. 31:193-196 . PubMed
  9. Tada T, et al. 2020. Cell Rep. 33:108528. PubMed
  10. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  11. Wagner W, et al. 2019. Cell Rep. 28:11. PubMed
  12. Antonyshyn JA, et al. 2021. Commun Biol. 4:1205. PubMed
RRID
AB_2563308 (BioLegend Cat. No. 405326)

Other Formats

View All IgG Reagents Request Custom Conjugation
Description Clone Applications
APC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Biotin Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
FITC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
HRP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,IHC,WB
PE Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Purified Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,FC,WB,IHC
Cyanine3 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
PE/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP/Cyanine5.5 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC,IHC-F,IHC-P
APC/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 421™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-F
Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
Brilliant Violet 605™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 510™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PE/Dazzle™ 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
APC/Fire™ 750 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Spark Red™ 718 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 IHC-P,IHC-F,ICC
Go To Top Version: 3    Revision Date: 10/28/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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