- Clone
- Poly4053 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Goat Polyclonal IgG
-
Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone UCHT1) anibody followed by anti-mouse IgG (clone Poly4053) Brilliant Violet 510™ (filled histogram). Unstained cells are represented by the open histogram.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405331 | 50 µg | $275.00 | |||
This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
-
Application References
(PubMed link indicates BioLegend citation) -
- Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
- Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
- Winters T, et al. 2014. EMBO J. 33:1256-70.
- Product Citations
-
- RRID
-
AB_2616858 (BioLegend Cat. No. 405331)
Antigen Details
- Distribution
-
B cells
- Gene ID
- 16059 View all products for this Gene ID
- UniProt
- View information about IgG on UniProt.org
Other Formats
View All IgG Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC Goat anti-mouse IgG (minimal x-reactivity)
-
Biotin Goat anti-mouse IgG (minimal x-reactivity)
-
FITC Goat anti-mouse IgG (minimal x-reactivity)
-
HRP Goat anti-mouse IgG (minimal x-reactivity)
-
PE Goat anti-mouse IgG (minimal x-reactivity)
-
Purified Goat anti-mouse IgG (minimal x-reactivity)
-
Cyanine3 Goat anti-mouse IgG (minimal x-reactivity)
-
PE/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes stained with purified mou... -
PerCP/Cyanine5.5 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes stained with purified mou... -
DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity)
Hela cells were stained with anti-Tubulin-γ and secondarily ... -
DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity)
-
Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity)
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ... 
Frozen human tonsil section was fixed with 4% paraformaldehy... 
Human paraffin-embedded placenta tissue slices were prepared... -
APC/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes stained with purified mou... -
Brilliant Violet 421™ Goat anti-mouse IgG (minimal x-reactivity)
293E cells were stained with purified anti-tubulin-alpha (cl... 
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity)
Frozen mouse intestine stained with purified anti-Ankyrin-B ... -
Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity)
Human paraffin-embedded tonsil tissue slices were prepared w... -
Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity)
HUVEC cells were fixed with 1% paraformaldehyde (PFA), and t... -
Brilliant Violet 605™ Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes were stained with purifie... -
Brilliant Violet 510™ Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes were stained with purifie... -
PE/Dazzle™ 594 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes were stained with purifie... -
PerCP Goat anti-mouse IgG (minimal x-reactivity)
-
APC/Fire™ 750 Goat anti-mouse IgG (minimal x-reactivity)
-
Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity)
Human peripheral blood lymphocytes stained with purified ant... 
Human paraffin-embedded tonsil tissue slices were prepared w... -
Spark Red™ 718 Goat anti-mouse IgG (minimal x-reactivity)
IHC staining of Spark Red™ 718 Goat anti-mouse IgG (clone Po... 
IHC staining of Spark Red™ 718 Goat anti-mouse IgG (clone Po... 
HeLa cells were fixed with Fixation Buffer (Cat. No. 420801)...