Alexa Fluor® 488 Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Poly4054_A488_IgG_IHC-F_091018.png
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of purified anti-mouse/human CD45R/B220 (clone RA3-6B2) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (red) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 488 (green) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • Poly4054_A488_IgG_IHC-F_091018.png
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of purified anti-mouse/human CD45R/B220 (clone RA3-6B2) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (red) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 488 (green) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 488 spectral data
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405418 100 µg $188.00
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
  3. Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations
  1. Fowler FC, et al. 2022. Elife. 11: . PubMed
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  3. Chen BR, et al. 2022. Bio Protoc. 12:e4413. PubMed
  4. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  5. Koop K, et al. 2023. Front Immunol. 14:1163198. PubMed
  6. Song JW, et al. 2021. Theranostics. 11:8874. PubMed
  7. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  8. Coleman LG, et al. 2018. Brain Behav Immun. 72:61. PubMed
  9. Garcia GL, et al. 2020. Am J Pathol. 543:190. PubMed
  10. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  11. Lisk C, et al. 2020. Front Immunol. 1.329166667. PubMed
  12. Chen BR, et al. 2021. Elife. 10:. PubMed
  13. Blackshear CP, et al. 2018. Tissue Eng Part C Methods. 24:566. PubMed
  14. Desbien A, et al. 2016. J Immunol. 197:4351-4359. PubMed
  15. Mead BE et al. 2018. BMC biology. 16(1):62 . PubMed
  16. Huang L, et al. 2016. Cell Death Dis. 7:e2301. PubMed
  17. Naito H, et al. 2019. Dev Cell. 48:151. PubMed
RRID
AB_2563120 (BioLegend Cat. No. 405418)
Go To Top Version: 4    Revision Date: 11/11/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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