Alexa Fluor® 594 Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Poly4054_A594_IgG_Antibody_1_100219
NIH/3T3 mouse fibroblast cell line was fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/mL purified anti-mouse/human CD44 (clone IM7) at room temperature for 3 hours, followed by 2.5 µg/mL anti-rat IgG (clone Poly4054) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). Image was captured with a 20X objective.
  • Poly4054_A594_IgG_Antibody_1_100219
    NIH/3T3 mouse fibroblast cell line was fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/mL purified anti-mouse/human CD44 (clone IM7) at room temperature for 3 hours, followed by 2.5 µg/mL anti-rat IgG (clone Poly4054) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). Image was captured with a 20X objective.
  • Poly4054_A594_IgG_Antibody_2_100219
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse B220 (clone RA3-6B2), and anti-mouse CD3ε (clone 145-2C11) Alexa Fluor® 647 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Check Availability
405422 100 µg $188.00
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
  3. Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations
  1. Fowler FC, et al. 2022. Elife. 11: . PubMed
  2. Fernández-Albarral JA, et al. 2022. Antioxidants (Basel). 11: . PubMed
  3. Smith MH, et al. 2023. Nat Immunol. . PubMed
  4. Lee YS, et al. 2018. Cell Host Microbe. 24:833. PubMed
RRID
AB_2563301 (BioLegend Cat. No. 405422)
Go To Top Version: 3    Revision Date: 10/02/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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