Alexa Fluor® 647 Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Poly4054_A647_IgG_Antibody_1_111120
Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-human CD312 (clone W15101A) and 10 µg/ml of Brilliant Violet 421™ anti-human CD19 (clone SJ25C1) (blue) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 647 Goat anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. The image was captured by 10X objective.
  • Poly4054_A647_IgG_Antibody_1_111120
    Human frozen tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-human CD312 (clone W15101A) and 10 µg/ml of Brilliant Violet 421™ anti-human CD19 (clone SJ25C1) (blue) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 647 Goat anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
405416 100 µg $188.00
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
  3. Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations
  1. Zhang Z, et al. 2022. Cancer Gene Ther. 29:722. PubMed
  2. Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed
  3. Yu L, et al. 2022. Cancer Sci. 113:3980. PubMed
  4. Ghanei Z, et al. 2020. Journal of Cellular Physiology. 235:7840. PubMed
  5. Kim H, et al. 2020. ACS Nano. 14:6519. PubMed
  6. Duan H, et al. 2020. JCI Insight. 5:00. PubMed
  7. Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed
  8. Zhang P, et al. 2020. Nat Commun. 11:2487. PubMed
  9. Hu J, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.72. PubMed
RRID
AB_2562967 (BioLegend Cat. No. 405416)
Go To Top Version: 4    Revision Date: 11/11/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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