Alexa Fluor® 594 anti-human CD45RB Antibody

Pricing & Availability
Clone
MEM-55 (See other available formats)
Regulatory Status
RUO
Workshop
V CD45.08
Other Names
CD45RB
Isotype
Mouse IgG2b, κ
MEM-55_A594_CD45RB_Antibody_032718
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10 mM, pH 6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 goat anti-CD45RB (clone MEM-55) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • MEM-55_A594_CD45RB_Antibody_032718
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10 mM, pH 6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 goat anti-CD45RB (clone MEM-55) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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310207 25 µg $136.00
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310208 100 µg $375.00
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Description

CD45RB is a 180-240 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines. The MEM-55 antibody recognizes a silaidase-sensitive epitope of CD45.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human thymocytes and T lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The MEM-55 antibody is specific for a glycosylated-CD45RB epitope that is highly expressed on memory B cells and plasmablasts but not on naïve B cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Horejsi V, et al. 1988. Folia Biol. 34:23.
  2. Bazil V, et al. 1989. Immunogenetics 29:202.
  3. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  4. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  5. Koethe S, et al. 2011. J. Leukoc. Biol. 90:5.
RRID
AB_2734281 (BioLegend Cat. No. 310207)
AB_2734281 (BioLegend Cat. No. 310208)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 180-240 kD (exon 5 splicing of CD45 gene)
Distribution

Thymocytes, T cell subset, B cells, monocytes/macrophages, granulocytes, dendritic cells

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RB on UniProt.org
Go To Top Version: 1    Revision Date: 04/02/2018

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