Alexa Fluor® 647 anti-mouse CD184 (CXCR4) Antibody

Pricing & Availability
Clone
L276F12 (See other available formats)
Regulatory Status
RUO
Other Names
Fusin, LESTR, Sdf1r, Cmkar4, PB-CKR, PBSF/SDF-1
Isotype
Rat IgG2b, κ
L276F12_AF647_CD184_Antibody_FC_1_093013
C57BL/6 mouse thymocytes were stained with CD4 FITC and CD184 (clone L276F12) Alexa Fluor® 647 (top) or rat IgG2b, κ Alexa Fluor® 647 isotype control (bottom).
  • L276F12_AF647_CD184_Antibody_FC_1_093013
    C57BL/6 mouse thymocytes were stained with CD4 FITC and CD184 (clone L276F12) Alexa Fluor® 647 (top) or rat IgG2b, κ Alexa Fluor® 647 isotype control (bottom).
  • L276F12_AF647_CD184_Antibody_FC_2_093013
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
146503 25 µg $147.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
146504 100 µg $347.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD184, also known as CXCR4, is a member of the G protein coupled receptor family that binds CXCL12 (SDF1). CXCR4 and CXCL12 play an important role in immune and inflammatory responses through the regulation of cell migration and growth. CXCR4 plays a crucial role in the pathogenesis of several autoimmune diseases such as atherosclerosis, rheumatoid arthritis, and wound healing. In addition, CXCR4 is the cofactor for fusion and entry of the T cell-tropic form of HIV-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CXCR4-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo blocking1

Application References

(PubMed link indicates BioLegend citation)
  1. Costa MJ, et al. 2018. PLoS One. 13:e0194688 (Block) PubMed
Product Citations
  1. Tundidor I, et al. 2023. Nat Commun. 14:3130. PubMed
  2. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  3. Iinuma S, et al. 2015. J Immunol. 194:1996. PubMed
  4. Gao X, et al. 2021. Nature. 589:591. PubMed
  5. Penzo M, et al. 2014. Biochim Biophys Acta. 1843:1796. PubMed
RRID
AB_2562590 (BioLegend Cat. No. 146503)
AB_2562590 (BioLegend Cat. No. 146504)

Antigen Details

Structure
G-protein-coupled seven transmembrane receptor, 39.7 kD
Distribution

T lymphocytes, monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, vascular smooth muscle cells (VSMCs)

Function
Development of cardiovascular and central nervous system, hematopoiesis, and colonization of BM by fetal liver-derived hematopoietic stem cells (HSCs) during embryogenesis, homing and egress of CD34+ CXCR4+ progenitor cells from bone marrow, and their migration into peripheral tissues. Critical role in homing of cancer cells to specific metastatic sites. Possible role in the ubiquitin proteosome system.
Interaction
Robo -1
Ligand/Receptor
CXCL12 (SDF-1)
Cell Type
Macrophages, Mast cells, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Ubiquitin/Protein Degradation
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kucia M, et al. 2005. Stem Cells 23:879.
2. Muller A, et al. 2001. Nature 410:50.
3. Saini V, et al. 2010. J. Biol. Chem. 285:15566.
4. Prasad A, et al. 2007. J. Leuko. Biol. 82:465. 
5. De Klerck B, et al. 2005. Arthritis Res. Ther. 7:R1208.
6. Rueda P, et al. 2008. PLoS One 3:e2543. 
7. Feng Y, et al. 1996. Science 272:872.

Gene ID
12767 View all products for this Gene ID
UniProt
View information about CD184 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account