Brilliant Violet 421™ anti-mouse CD184 (CXCR4) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CXCR4-transfected cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vivo blocking¹

Application References

(PubMed link indicates BioLegend citation)

1. Costa MJ, et al. 2018. PLoS One. 13:e0194688 (Block) PubMed

Product Citations

1. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
2. Brulois K, et al. 2020. Nat Commun. 3.095833333. PubMed
3. Matter AL, et al. 2022. J Immunol. 209:1880. PubMed
4. Stoler-Barak L, et al. 2023. Nat Commun. 14:1462. PubMed
5. del Rio ML, et al. 2023. Front Immunol. 14:1113858. PubMed
6. Girousse A et al. 2019. Cell Rep. 27(2):323-333 . PubMed
7. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
8. Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed
9. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
10. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
11. Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed
12. Beidaq A, et al. 2016. J Immunol. 197: 1567 - 1576. PubMed
13. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
14. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
15. Subramaniam N, et al. 2021. Blood Adv. 5:1259. PubMed
16. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
17. Chen KE, et al. 2021. J Neuroinflammation. 18:140. PubMed

RRID

AB_2562788 (BioLegend Cat. No. 146511)

Antigen Details

Structure

G-protein-coupled seven transmembrane receptor, 39.7 kD

Distribution

T lymphocytes, monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, vascular smooth muscle cells (VSMCs)

Function

Development of cardiovascular and central nervous system, hematopoiesis, and colonization of BM by fetal liver-derived hematopoietic stem cells (HSCs) during embryogenesis, homing and egress of CD34⁺ CXCR4⁺ progenitor cells from bone marrow, and their migration into peripheral tissues. Critical role in homing of cancer cells to specific metastatic sites. Possible role in the ubiquitin proteosome system.

Interaction

Robo -1

Ligand/Receptor

CXCL12 (SDF-1)

Cell Type

Macrophages, Mast cells, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells

Biology Area

Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Ubiquitin/Protein Degradation

Molecular Family

CD Molecules, Cytokine/Chemokine Receptors, GPCR

Antigen References

1. Kucia M, et al. 2005. Stem Cells 23:879.
2. Muller A, et al. 2001. Nature 410:50.
3. Saini V, et al. 2010. J. Biol. Chem. 285:15566.
4. Prasad A, et al. 2007. J. Leuko. Biol. 82:465. 
5. De Klerck B, et al. 2005. Arthritis Res. Ther. 7:R1208.
6. Rueda P, et al. 2008. PLoS One 3:e2543. 
7. Feng Y, et al. 1996. Science 272:872.

Gene ID

12767 View all products for this Gene ID

UniProt

View information about CD184 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats