Alexa Fluor® 647 anti-mouse CD9 Antibody

Pricing & Availability
Clone
MZ3 (See other available formats)
Regulatory Status
RUO
Other Names
DRAP-27, MRP-1, p-24
Isotype
Rat IgG2a, κ
MZ3_A647_CD9_Antibody_1_102324
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cyanine5.5 and MZ3 Alexa Fluor® 647
  • MZ3_A647_CD9_Antibody_1_102324
    C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cyanine5.5 and MZ3 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
124809 25 µg $124.00
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124810 100 µg $253.00
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Description

CD9 is a surface glycoprotein of the tetraspannin family. It is expressed on a variety of cells, including nerve, muscle cells and many cells of hematopoietic origin. CD9 is found to participate in forming a large molecular cell complex with other membrane proteins, such as MHC class II, CD19, CD5 and other TM4SF molecules. It is reported that CD9 is a marker of marginal zone B cells, B1 cells and plasma cells. The diverse functions of CD9 may largely depend upon its associated molecules on different cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Won WJ, et al. 2002. J. Immunol. 168:5605. (FC, WB)
Product Citations
  1. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  2. Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed
  3. Khan NZ, et al. 2021. Brain Behav Immun. 92:165. PubMed
  4. Zakeri A, et al. 2021. J Extracell Vesicles. 10:e12131. PubMed
  5. Li K, et al. 2018. Methods Mol Biol. 1740:69. PubMed
  6. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  7. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  8. Björck P, et al. 2011. J Immunol. 186:1477. PubMed
  9. Viveiros A, et al. 2022. Cells. 11:. PubMed
  10. Gyuris A et al. 2019. Cell Rep. 27(13):3972-3987 . PubMed
  11. Kitadate Y, et al. 2019. Cell Stem Cell. 24:79. PubMed
  12. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  13. Lindhorst A, et al. 2021. Cell Death Dis. 12:579. PubMed
  14. Patras L, et al. 2020. Int J Mol Sci. 21:00. PubMed
  15. Garbuzenko O, et al. 2010. Proc Natl Acad Sci U S A. 107:10737. PubMed
  16. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
RRID
AB_2076037 (BioLegend Cat. No. 124809)
AB_2076037 (BioLegend Cat. No. 124810)

Antigen Details

Structure
24 kd surface glycoprotein belonging to the tetraspanin (TM4SF)family which is characterized by four transmembrane-spanning domains and two extracellular domains.
Distribution

CD9 is expressed in nerve, muscle, keratinocytes, fibroblasts and a variety of hematopoietic cells including monocytes, macrophages, granulocytes , platelets and activated T and B cells.

Interaction
CD9 may participate in forming large molecular complexes with other associated molecules.
Cell Type
B cells, Embryonic Stem Cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, Platelets, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Boucheix C, et al. 1991. J. Biol. Chem. 266:117
2. Lanza F, et al. 1991. J. Biol. Chem. 266:10638

Gene ID
12527 View all products for this Gene ID
UniProt
View information about CD9 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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