Alexa Fluor® 647 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
A_11-26Cdot2a_A647_042909.jpg
C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647
  • A_11-26Cdot2a_A647_042909.jpg
    C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647
  • B_11-26cdot2a_A647_IgD_Antibody_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD68 Antibody (clone FA-11) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD3ε Antibody (clone 500A2) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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405707 25 µg $112.00
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405708 100 µg $265.00
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Description

Surface IgD is an important B cell differentiation marker.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  2. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  3. Gallman AE, et al. 2021. Sci Immunol. 6:. PubMed
  4. Chan T, et al. 2009. J Immunol. 183:3139. PubMed
  5. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  6. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  7. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  8. Shi B, et al. 2018. J Immunol. 200:586. PubMed
  9. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  10. Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed
  11. Gatto D, et al. 2011. J Immunol. 187:4621. PubMed
  12. Vu T, et al. 2011. J Immunol. 187:5032. PubMed
  13. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  14. Adori M, et al. 2018. J Immunol. 200:775. PubMed
  15. Avery DT, et al. 2018. J Exp Med. 215:2073. PubMed
  16. He Y, et al. 2022. J Exp Med. 219:. PubMed
  17. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  18. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  19. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  20. Ogbe A, et al. 2015. J Biol Chem. 290: 20455-20465. PubMed
  21. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  22. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  23. Zhong MC, et al. 2021. J Exp Med. 218:. PubMed
  24. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  25. Volkmann C, et al. 2016. EMBO J. 35: 2371 - 2381. PubMed
  26. Kishi Y, et al. 2012. Proc Natl Acad Sci U S A. 109:7811. PubMed
  27. Vacchio MS, et al. 2020. Immunity. 51(3):465-478. PubMed
  28. Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed
  29. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
  30. Fleig S, et al. 2022. Nat Commun. 13:2022. PubMed
  31. Cheng Q, et al. 2018. PLoS Pathog. 14:e1007440. PubMed
  32. Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
  33. Monzon-Casanova E, et al. 2021. Eur J Immunol. 51:2266. PubMed
  34. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  35. Lahoud M, et al. 2011. J Immunol. 187:842. PubMed
  36. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  37. Jandl C, et al. 2017. Nat Commun. 10.1038/ncomms14647. PubMed
  38. Maity P, et al. 2015. Sci Signal. 8: ra93. PubMed
  39. Dahlgren M, et al. 2015. J Immunol. 194:5187. PubMed
  40. Barwick BG, et al. 2018. Nat Commun. 9:1900. PubMed
RRID
AB_893529 (BioLegend Cat. No. 405707)
AB_893529 (BioLegend Cat. No. 405708)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 3    Revision Date: 09/21/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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