Alexa Fluor® 700 anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
HCD56_Alx700_043009.jpg
Human peripheral blood lymphocytes stained with CD16 (3G8) PE and HCD56 Alexa Fluor® 700
  • HCD56_Alx700_043009.jpg
    Human peripheral blood lymphocytes stained with CD16 (3G8) PE and HCD56 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
318315 25 µg $124.00
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318316 100 µg $265.00
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed
  2. Weskamm LM, et al. 2022. Cell Rep Med. 3:100685. PubMed
  3. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  4. Crespo ÂC, et al. 2020. Cell. 182(5):1125-1139. PubMed
  5. Catapano M, et al. 2020. J Invest Dermatol. 140:816. PubMed
  6. Cillo AR, et al. 2020. Immunity. 52(1):183-199.e9.. PubMed
  7. Coelho CH, et al. 2020. JCI Insight. 5:. PubMed
  8. McKinnon J, et al. 2014. PLoS One. 9:95524. PubMed
  9. Hearps AC, et al. 2017. Front Immunol. 0.840972222. PubMed
RRID
AB_604104 (BioLegend Cat. No. 318315)
AB_604104 (BioLegend Cat. No. 318316)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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