PE anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
HCD56_PE_022008
Human peripheral blood lymphocytes stained with HCD56 PE
  • HCD56_PE_022008
    Human peripheral blood lymphocytes stained with HCD56 PE
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318305 25 tests $122.00
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318306 100 tests $268.00
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
  2. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
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  4. Andonian BJ, et al. 2022. Sci Rep. 12:7450. PubMed
  5. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  6. Brusilovsky M, et al. 2013. J Immunol. 191:5256. PubMed
  7. Abbas Y, et al. 2017. Journal of the Royal Society Interface. 10.1098/rsif.2017.0131. PubMed
  8. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  9. Zhao Y, et al. 2021. Front Immunol. 12:665442. PubMed
  10. Armour K, et al. 2014. PLoS One. 9:109463. PubMed
  11. Seamon K, et al. 2020. Front Immunol. 11:396. PubMed
  12. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  13. Leem G, et al. 2021. J Allergy Clin Immunol. 148:996. PubMed
  14. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  15. Fionda C, et al. 2009. J Immunol. 183:4385. PubMed
  16. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  17. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  18. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  19. Sandström N, et al. 2022. Cell Rep Methods. 2:100256. PubMed
  20. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  21. Yin W, et al. 2021. Thorac Cancer. 12:2680. PubMed
  22. Hsu H, et al. 2016. J Immunol. 197: 1884 - 1892. PubMed
  23. Kuo HH, et al. 2018. Immunity. 48:1183. PubMed
  24. Walk J, et al. 2020. Front Immunol. 2.405555556. PubMed
  25. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  26. Gawel DR, et al. 2019. Genome Med. 11:47. PubMed
  27. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
  28. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  29. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  30. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  31. Elias S, et al. 2020. Haematologica. . PubMed
  32. Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed
  33. van Erp EA, et al. 2020. Clin Transl Immunology. 9:e1112. PubMed
  34. Warthan MD, et al. 2018. Biol Reprod. 98:309. PubMed
  35. Obiero J, et al. 2015. Infect Immun . 83:2185. PubMed
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RRID
AB_604101 (BioLegend Cat. No. 318305)
AB_604101 (BioLegend Cat. No. 318306)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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