Biotin anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
HCD56_Biotin_022008
Human peripheral blood lymphocytes stained with biotinylated HCD56, followed by Sav-PE
  • HCD56_Biotin_022008
    Human peripheral blood lymphocytes stained with biotinylated HCD56, followed by Sav-PE
  • HCD56-Biot_IF_101410
    Human NK-92 cells were stained with CD56 (clone HCD56) Biotin, and then secondarily stained with Streptavidin-Alexa Fluor® 488. Cells were imaged with a Zeiss Axio Observer Z1 spinning disc confocal, average exposure time ~0.23 seconds. Credit: Dr. Jordan Orange and Dr. Emily Mace, University of Pennsylvania School of Medicine Children's Hospital of Philadelphia, Division of Immunology.
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Cat # Size Price Quantity Check Availability
318319 25 µg $106.00
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318320 100 µg $211.00
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IF - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Benjamin DI, et al. 2022. Cell Metab. 34:902. PubMed
  2. Wei X, et al. 2022. Mol Cell. 82:1225. PubMed
  3. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  4. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  5. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  6. Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed
  7. Sarkar TJ, et al. 2020. Nat Commun. 11:1545. PubMed
  8. Quarta M, et al. 2016. Nat Biotechnol. 10.1038/nbt.3576. PubMed
  9. Goods BA, et al. 2021. JCI Insight. 6: . PubMed
  10. An X, et al. 2017. PLoS One.. 10.1371/journal.pone.0181904. PubMed
  11. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  12. Askenase MH, et al. 2021. Sci Immunol. 6:. PubMed
  13. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
RRID
AB_893390 (BioLegend Cat. No. 318319)
AB_893390 (BioLegend Cat. No. 318320)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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