Alexa Fluor® 700 anti-mouse CD90.2 (Thy-1.2) Antibody

Pricing & Availability
Clone
53-2.1 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1.2
Isotype
Rat IgG2a, κ
53-2-1_A700_CD90point2_Antibody_123015
C57BL/6 mouse splenocytes were stained with CD90.2 (clone 53-2.1) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
  • 53-2-1_A700_CD90point2_Antibody_123015
    C57BL/6 mouse splenocytes were stained with CD90.2 (clone 53-2.1) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
140323 25 µg $101.00
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140324 100 µg $288.00
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).

Application References

(PubMed link indicates BioLegend citation)
  1. Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
  2. Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
  3. Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
Product Citations
  1. Huseni MA, et al. 2023. Cell Rep Med. 4:100878. PubMed
  2. Hsieh E, et al. 2023. PLoS Pathog. 19:e1011101. PubMed
  3. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  4. Morehead LC, et al. 2023. Cancers (Basel). :15. PubMed
  5. Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
  6. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
RRID
AB_2566739 (BioLegend Cat. No. 140323)
AB_2566739 (BioLegend Cat. No. 140324)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org
Go To Top Version: 1    Revision Date: 12/31/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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