Brilliant Violet 510™ anti-mouse CD90.2 (Thy-1.2) Antibody

Pricing & Availability
Clone
53-2.1 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1.2
Isotype
Rat IgG2a, κ
2.1_BV510_CD90.2_Antibody_FC_091112
C57BL/6 mouse splenocytes were stained with CD90.2 (clone 53-2.1) Brilliant Violet 510™ (filled histogram) or rat IgG2a, κ Brilliant Violet 510™ isotype control (open histogram).
  • 2.1_BV510_CD90.2_Antibody_FC_091112
    C57BL/6 mouse splenocytes were stained with CD90.2 (clone 53-2.1) Brilliant Violet 510™ (filled histogram) or rat IgG2a, κ Brilliant Violet 510™ isotype control (open histogram).
Compare all formats See Brilliant Violet 510™ spectral data
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140319 125 µL $165.00
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).

Application References

(PubMed link indicates BioLegend citation)
  1. Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
  2. Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
  3. Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
Product Citations
  1. Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed
  2. Xu W 2023. Cell Reports. 42(1):111987. PubMed
  3. Sakae H, et al. 2023. Cells. 12:. PubMed
  4. Georges RO, et al. 2022. Nat Commun. 13:6230. PubMed
  5. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  6. Vicetti Miguel R, et al. 2016. PLoS One. 11: 0162445. PubMed
  7. Wang H, et al. 2022. Curr Protoc. 2:e446. PubMed
  8. Place D, et al. 2017. PLoS One. 10.1371/journal.pone.0190384. PubMed
  9. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  10. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  11. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  12. Baerenwaldt A, et al. 2016. J Immunol. 196: 2561 - 2571. PubMed
  13. Krisna SS, et al. 2021. STAR Protoc. 2:100627. PubMed
  14. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
RRID
AB_2561395 (BioLegend Cat. No. 140319)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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