APC/Cyanine7 anti-mouse CD90.2 (Thy1.2) Antibody

Pricing & Availability
Clone
53-2.1 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1.2
Isotype
Rat IgG2a, κ
53-2dot1_APCCy7_CD90dot2_Thy-1dot2_Antibody_082521.png
C57BL/6 mouse splenocytes were stained with Alexa Fluor® 488 CD3 and CD90.2 (clone 53-2.1) APC/Cyanine7 (left) or Rat IgG2a, κ isotype control (clone RTK2758) APC/Cyanine7 (right).
  • 53-2dot1_APCCy7_CD90dot2_Thy-1dot2_Antibody_082521.png
    C57BL/6 mouse splenocytes were stained with Alexa Fluor® 488 CD3 and CD90.2 (clone 53-2.1) APC/Cyanine7 (left) or Rat IgG2a, κ isotype control (clone RTK2758) APC/Cyanine7 (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
140331 25 µg $118.00
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140332 100 µg $323.00
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).

Application References

(PubMed link indicates BioLegend citation)
  1. Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
  2. Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
  3. Okada C, et al. 1990. J. Immunol. 144:3473. (IP)
Product Citations
  1. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  2. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
RRID
AB_2894662 (BioLegend Cat. No. 140331)
AB_2894662 (BioLegend Cat. No. 140332)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org
Go To Top Version: 1    Revision Date: 08/25/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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