APC anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
UCHT1_APC_021805
Human peripheral blood lymphocytes stained with UCHT1 APC
  • UCHT1_APC_021805
    Human peripheral blood lymphocytes stained with UCHT1 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability
300411 25 tests $33.00
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300412 100 tests $72.00
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300458 100 µg $78.00
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300439 500 tests $264.00
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Vazquez-Lombardi R, et al. 2022. Immunity. 55:1953. PubMed
  3. Wheeler MM, et al. 2022. Nat Commun. 13:7592. PubMed
  4. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  5. Yen M, et al. 2022. Cell. 185:1414. PubMed
  6. Crawford MP, et al. 2020. Proc Natl Acad Sci U S A. 117:19408. PubMed
  7. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
  8. Zhang Y, et al. 2023. Heliyon. 9:e14823. PubMed
  9. Crawford MP, et al. 2023. PLoS One. 18:e0285166. PubMed
  10. Jiang D, et al. 2023. Nat Commun. 14:3642. PubMed
  11. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  12. Martin–Fernandez M, et al. 2020. Cell Reports. 31(6):107633. PubMed
  13. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  14. Gutzeit C, et al. 2014. J Immunol. 192:5852. PubMed
  15. Sun L, et al. 2020. J Diabetes Res. 2020:2583257. PubMed
  16. Ogishi M, et al. 2021. Nat Med. 27:1646. PubMed
  17. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  18. Paris O, et al. 2022. Front Immunol. 13:975910. PubMed
  19. Wang C, et al. 2021. Cell Stem Cell. . PubMed
  20. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  21. Lichtenegger FS, et al. 2018. Front Immunol. 9:385. PubMed
  22. Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed
  23. Constantinescu-Bercu A, et al. 2020. eLife. 9:00. PubMed
  24. Gerstner S, et al. 2016. J Leukoc Biol. 100(6):1297-1310. PubMed
  25. Levring TB, et al. 2019. Sci Rep. 9:16725. PubMed
  26. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  27. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  28. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  29. Helmink BA, et al. 2020. Nature. 577:549. PubMed
  30. Hu H, et al. 2022. Exp Ther Med. 24:487. PubMed
  31. Jing L, et al. 2020. Cancer Immunol Res. 8:648. PubMed
  32. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  33. Huang GL, et al. 2020. PLoS One. 15:e0241421. PubMed
  34. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  35. Meraviglia S, et al. 2017. Oncoimmunology. 6:e1347742. PubMed
  36. Fujii S, et al. 2019. Stem Cells Dev. 28:28. PubMed
  37. Ling X, et al. 2022. STAR Protoc. 3:101321. PubMed
  38. Hannon E, et al. 2021. PLoS Genet. e1009443:17. PubMed
  39. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  40. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  41. Kim JR, et al. 2005. BMC Immunol. 2005,6:3. PubMed
  42. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  43. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  44. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  45. Zhang B, et al. 2020. Cell Chemical Biology. 28(1):60-69.e7. PubMed
  46. Morton B, et al. 2021. Nat Commun. 12:3554. PubMed
  47. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  48. Singh KS, et al. 2021. Nature. 589:597. PubMed
  49. Barresi V, et al. 2020. J Clin Med. 9:00. PubMed
  50. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  51. Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
  52. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
  53. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  54. Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
  55. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  56. Lo Presti E, et al. 2020. J Leukoc Biol. 108:749. PubMed
  57. Headland S, et al. 2014. Sci Rep. 4:5237. PubMed
  58. Giron LB, et al. 2020. EBioMedicine. 73.94791667. PubMed
  59. Siegers G, et al. 2011. PLoS One. 6:e16700. PubMed
  60. Anderson NR, et al. 2019. Cell Adh Migr. 13:163. PubMed
RRID
AB_2562045 (BioLegend Cat. No. 300411)
AB_2562045 (BioLegend Cat. No. 300412)
AB_2562045 (BioLegend Cat. No. 300458)
AB_2562045 (BioLegend Cat. No. 300439)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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