APC anti-mouse IgG1 Antibody

Pricing & Availability
Clone
RMG1-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G1
Isotype
Rat IgG
RMG1-1_APC_111710
Human peripheral blood lymphocytes were stained with purified mouse IgG1 anti-human CD3 (clone UCHT1, filled histogram) or mouse IgG1, k isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) APC.
  • RMG1-1_APC_111710
    Human peripheral blood lymphocytes were stained with purified mouse IgG1 anti-human CD3 (clone UCHT1, filled histogram) or mouse IgG1, k isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) APC.
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Cat # Size Price Quantity Check Availability
406609 50 µg $113.00
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406610 200 µg $326.00
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Description

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed
  2. Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations
  1. Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
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  4. Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed
  5. Ehling RA, et al. 2022. Cell Rep. 38:110242. PubMed
  6. Ishii M, et al. 2021. Pharmaceutics. 13:. PubMed
  7. Bhattacharya Y 2014. J Exp Med. 211:841. PubMed
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  9. Manresa MC, et al. 2020. Gastroenterology. 159:1778. PubMed
  10. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  11. Duan L, et al. 2021. Immunity. 54:2256. PubMed
  12. Günal-Sadık G, et al. 2014. PLoS One. 9:111748. PubMed
  13. Hare DN, et al. 2021. STAR Protoc. 2:100436. PubMed
  14. De Veirman K, et al. 2021. Oncoimmunology. 10:2000699. PubMed
  15. Gradiz R, et al. 2016. Sci Rep. 6:21648. PubMed
  16. Vergadi E, et al. 2014. J Immunol. 192:394. PubMed
  17. Kim S, et al. 2021. Animals (Basel). 11:. PubMed
  18. Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed
  19. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  20. Deen D, et al. 2021. Blood Adv. 5:3002. PubMed
  21. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  22. Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed
  23. Stokes K, et al. 2013. J Virol. 87:10070. PubMed
  24. Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
  25. Roberts JT, et al. 2020. Mol Cell Proteomics. 19:362. PubMed
  26. Jang A, et al. 2021. Front Immunol. 12:701066. PubMed
  27. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  28. Cronin J, et al. 2016. Mucosal Immunol. 10.1038/mi.2015.131. PubMed
RRID
AB_10679040 (BioLegend Cat. No. 406609)
AB_10679040 (BioLegend Cat. No. 406610)
Go To Top Version: 2    Revision Date: 01/29/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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